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A Comparison of Results Obtained by Flow Microfluorometric and Autoradiographic Method

山口医学 Volume 30 Issue 3 Page 227-232
published_at 1981-09
Title
L5178Y細胞のCell Cycle 解析 Flow CytometryとOkada法による解析結果の比較
A Comparison of Results Obtained by Flow Microfluorometric and Autoradiographic Method
Creators Ishiguro Kimio
Source Identifiers
Growth characteristics of L5178Y cells (mouse leukemic cells in vitro) in exponential phase were analyzed by the combination of methods by Puck and Steffen and by Okada and the cell cycle parameters were compared with tbose obtained by flow microfluorometirc measurement of DNA content distribution. The durations of G1, S, G2, and M were 2.2, 7.8, 1.8, and 0.7 hours, respectively. The growth fraction equaled unity. The fractions of cells in G1, S, and G2+M phases obtained by the analtsis of DNA content distribution pattern were 25.47, 62.73 and 11.80% and showed a good approximation to those estimated from kinetic analysis (G1=23.42% S=61.71%, G2=10.75%, and M=4.12%) . The coefficient of variation was 4.4%…
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1981-09
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部