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Indirect hemagglutination test for diagnosis of canine filariasis

The Japanese journal of veterinary science Volume 43 Issue 1 Page 21-26
published_at 1981
2008010289.pdf
[fulltext] 521 KB
Title
Indirect hemagglutination test for diagnosis of canine filariasis
Creators Hayasaki Mineo
Four kinds of Dirofilaria immitis antigens, prepared from intrauterine micro-filariae (I-Mf), circulating microfilariae (C-Mf), migrating larvae and adult worms (A-Di), were assessed by indirect hemagglutination (IHA) test in terms of immunological specificity and sensitivity. The highest specificity and sensitivity were observed in phosphate buffered saline (PBS) extract of I-Mf among these antigens. In contrast, PBS extract of C-Mf was found to have extremely low IHA antigenicity. Cross reactions between D. immitis and some intestinal parasites, such as Toxocara canis, Ancylostoma caninum and Trichuris vulpis, were evaluated by agglutinin absorption assay. It was revealed that no substantial cross reaction was present between I-Mf or A-Di and the above three intestinal parasites. Agglutinin absorption assay also suggested that the antigenicity of A-Di antigen differed from that of I-Mf antigen, although some common antigens were present between them. The present results indicate that I-Mf antigen is useful for IHA test in D. immitis infection in dogs.
Languages eng
Resource Type journal article
Publishers 日本獣医学会
Date Issued 1981
File Version Version of Record
Access Rights open access
Relations
[ISSN]0021-5295
[NCID]AN00191788
[isVersionOf] [URI]http://www.jstage.jst.go.jp/browse/jvms/-char/ja
[isVersionOf] Dirofilaria immitis infection and dirofilariosis : an animal model of human filariosis [URI]http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2010010511
Schools 農学部