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Patjological Study on Amyloidosis Isolation and Purification of Amyroid Fibril Protein and Biochemical and Immunological Analysis

山口医学 Volume 30 Issue 2 Page 149-162
published_at 1981-06
Title
アミロイド症の病理学的研究 アミロイド蛋白の分離精製、分析および抗血清の作製
Patjological Study on Amyloidosis Isolation and Purification of Amyroid Fibril Protein and Biochemical and Immunological Analysis
Creators Imada Naoki
Source Identifiers
Examined specimens were obtained from eight patients of primary amyloidosis and two kinds of animals with amyloidosis, a swan and ICR mice. Isolation procedures of amyloid fibril proteins were performed according to the methods of Pras et al. (1968) and Glenner et al. (1972). After tissues were washed with PBS , crude amyloid fibrils extracted with water were fractionated by gel filtration on Sephadex G-100 column using 5 M guanidine-HCl. Collagenase treatment on crude amyloid, SDS electrophoresis, animo acid analysis of purified amyloid Protein AA and murine amyloid protein obtained by inocluating into rabbits were purified by passage through immunoabsorbent column…
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1981-06
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部