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Reverse genetics system for Tick-borne encephalitis virus using Circular Polymerase Extension Reaction

Infection, Genetics and Evolution Volume 132
published_at 2025-06-03
1-s2.0-S1567134825000656-main.pdf
[fulltext] 3.07 MB
Supplemental Data : https://public.oa.kenkyu.yamaguchi-u.ac.jp/4df44d5a-608d-4aaa-97c7-37a2be42c063
Title
Reverse genetics system for Tick-borne encephalitis virus using Circular Polymerase Extension Reaction
Creators Mitsunaga Saki
Creators Tamura Tomokazu
Creators Nyampong Samuel
Creators Fukuhara Takasuke
Creators Shimoda Hiroshi
Affiliate Master Yamaguchi University
[kakenhi]15501 grid.268397.1
Creators Hayasaka Daisuke
Affiliate Master Yamaguchi University
[kakenhi]15501 grid.268397.1
Source Identifiers [EISSN] 1567-7257
Creator Keywords
Tick-borne encephalitis virus Polymerase chain reaction-based Circular Polymerase Extension Reaction Reverse genetics system
Languages eng
Resource Type journal article
Publishers Elsevier
Date Issued 2025-06-03
Rights
© 2025 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC license.()
Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by-nc/4.0/)
File Version Version of Record
Access Rights open access
Relations
[isIdenticalTo] https://doi.org/10.1016/j.meegid.2025.105776
[isSupplementedBy] [URI]https://public.oa.kenkyu.yamaguchi-u.ac.jp/4df44d5a-608d-4aaa-97c7-37a2be42c063
Funding Refs
Japan Society for the Promotion of Science [crossref_funder]https://doi.org/10.13039/501100001691
Award Epidemiological surveys of tick-borne pathogens in Kenya 17H04661
Funding Refs
Japan Society for the Promotion of Science [crossref_funder]https://doi.org/10.13039/501100001691
Award Promotion of emerging and re-emerging virus research using high-speed reverse genetics 21H02736
Funding Refs
Japan Society for the Promotion of Science [crossref_funder]https://doi.org/10.13039/501100001691
Award Promotion of comprehensive interdisciplinary virology for the post-COVID era 23K20041
Funding Refs
Japan Society for the Promotion of Science [crossref_funder]https://doi.org/10.13039/501100001691
Award Determination of viral factors responsible to high pathogenicity of Severe Fever and Thrombocytopenia 24K01930
Funding Refs
Japan Society for the Promotion of Science [crossref_funder]https://doi.org/10.13039/501100001691
Award Elucidation of mechanism of Flavivirus NS1 secretion 24K09259
Funding Refs
Research Center for Thermotolerant Microbial Resources (RCTMR), Yamaguchi University
Award フラビウイルスの感染宿主動物と組織の解明に向けた基礎研究 2023-19
Funding Refs
Research Center for Thermotolerant Microbial Resources (RCTMR), Yamaguchi University
Award フラビウイルスの感染宿主動物と組織指向性の解明に向けた基礎研究 Ⅱ 2024-06
Funding Refs
Institute of Tropical Medicine, Nagasaki University
Award ケニアにおけるマダニ媒介感染症の疫学調査 2022-Kyoten-03
Funding Refs
Institute of Tropical Medicine, Nagasaki University
Award ケニアにおけるマダニ媒介感染症の疫学調査 2023-Kyoten-02
Funding Refs
Institute of Tropical Medicine, Nagasaki University
Award ケニアにおけるマダニ媒介感染症の疫学調査 2024-Kyoten-05
Funding Refs
Japan Agency for Medical Research and Development
Award 全てのRNAウイルスに対応可能な汎用的高速リバースジェネティクスの確立 21fk0108617h0001
Funding Refs
Japan Agency for Medical Research and Development
Award レポーター技術を駆使したデング熱新規診断法の開発 22fk0108563h0001
Funding Refs
Japan Agency for Medical Research and Development
Award 革新的生体イメージング技術によるフラビウイルス感染動態を解析できる動物モデルの開発 23fk0108598h0001
Funding Refs
Japan Agency for Medical Research and Development
Award 節足動物媒介性フラビウイルスの感染動態アトラスの作成 24wm0325073h0001
Funding Refs
Japan Agency for Medical Research and Development
Award ワクチン開発のための世界トップレベル研究開発拠群 北海道シナジーキャンパス(北海道大学 ワクチン研究開発拠点) 223fa627005h0001
Funding Refs
Japan Agency for Medical Research and Development
Award 革新的リバースジェネティクスを駆使した新たなウイルス学研究の創出 22gm1610008h0001