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Analysis of Neutral Glycolipids and Sulfatide in Human Colorectal Cancer

山口医学 Volume 45 Issue 1 Page 47-58
published_at 1996-02
Title
ヒト大腸癌の糖脂質分析 : スルファチドを中心として
Analysis of Neutral Glycolipids and Sulfatide in Human Colorectal Cancer
Creators Morichika Hiroshi
Source Identifiers
Creator Keywords
糖脂質 スルファチド 大腸癌 薄層クロマトグラフィー 免疫染色
Glycolipids are cellular membrane components that may play an important role in the development, differentiation, proliferation, and carcinogenesis of cells. Qualitative analysis of glycolipids and sulfatide in 22 primary colorectal cancer and 6 adjacent normal mucosa was performed using Thin Layer Chromatography (TLC) and TLC immuno-staining. Quantitative analysis was performed by a densitometric scanning method. Ceramide monohexoside (CMH) was significantly decreased and ceramide dihexoside (CDH) was significantly increased in cancerous tissues compared with normal ones (P<0.001). MD ratio [CMH / (CMH + CDH)] in 14 manifested lymph node metastasis was higher than in 7 which the above was absent (P< 0.05). And also in Dukes stage, MD ratio in 9 Dukes C was higher than in 7 Dukes A(P<0.05). Two spots of sulfatide (CSE) were observed with TLC immuno-staining method and we designated each spot as CSE-A and CSE-B. CSE-A was significantly decreased and CSE-B was significantly increased in cancerous tissues compared with normal tissues (P<0.05). CSE ratio [CSE-B / (CSE-A+ CSE-B)] in 15 lymph node metastasis cases was higher than that of 7 cases lacking metastasis(P<0.05). And also in Dukes stage, CSE ratio in 9 Dukes C and 6 Dukes D were higher than in 7 Dukes A(P<0.02, P<0.05). Change of glycolipids on the cellular membrane, especially change of sulfatide, were correlated with malignancy potential of colorectal cancer.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1996-02
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部