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Specium Method for Electorn Microscopy PartII. On the Supporting Membranes of Samples 3. A New Method with Highly Efficient Techniques for Membrane Making

山口医学 Volume 26 Issue 3 Page 189-195
published_at 1977-12
Title
走査電子顕微鏡標本調整法の研究 第二編 試料支持膜に関する研究 第三報 高能率支持膜作成法(新法)について
Specium Method for Electorn Microscopy PartII. On the Supporting Membranes of Samples 3. A New Method with Highly Efficient Techniques for Membrane Making
Creators Yoshii Zensaku
Creators Tanaka Syuhei
Creators Konishi Hisanori
Creators Okusa Akio
Creators Takamura Akira
Creators Kobayashi Masaru
Creators Iwasaki Akira
Source Identifiers
A highly efficient method of membrane making for the specimen preparaiton in transmission electron microscopy was contrived. The method includes the following procedures of techniques: 1) Spreading of a collodion film on the water surface 2) Regular placing of copper grids on the collodion film 3) Overlapping of a round filter paper on the above 4)Sinking of the filter paper into the water of vessel 5) Salvaging of the above and transferring into a Petri dish 6) Drying,carbon-coating and preservation of the filter paper. This method provides much more advantageous effects comparison with the conventional method. A large number of grids can be handled at a time of preparation and the following treatments, such as mount of samples, shadowing and negative staining , are very convenient. Therefore, this would be emphasized as a new method in the membrance making.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1977-12
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部