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Down-regulation of Ras / Akt Signaling and Hippocampal Neuronal Death following Transient Global Ischemia-Reperfusion in Rat brain

The bulletin of the Yamaguchi Medical School Volume 53 Issue 1-2 Page 5-12
published_at 2006
A050053000102.pdf
[fulltext] 776 KB
Title
Down-regulation of Ras / Akt Signaling and Hippocampal Neuronal Death following Transient Global Ischemia-Reperfusion in Rat brain
Creators Ying jun Cui
Creators Fukuda Shiro
Creators Hirata Takao
Creators Matayoshi Hiroaki
Creators Matsumoto Mishiya
Creators Sakabe Takefumi
Source Identifiers
Creator Keywords
Ras Akt ischemia-reperfusion hippocampus apoptosis
Apotosis is one of the components of delayed neuronal death in the hippocampal CA1 following transient cerebral ischemia. However, the precise signal transduction for inducing this type of death is not fully determined. Recent research has shown that oncogenis Ras protein plays a role in regulation of cell survival and death. We hypothesized that apoptosis in delayed neuronal death is regulated with Ras and its signaling cascades. We examined the temporal profile of Ras and its downstream cascade following transient forebrain ischemia (15 min) in rat brain. Neuronal death was inconsistetly observed in the CA1 of the hippocampus (HE staining) 2 days after ischemia, whereas most neurons in the CA1 showed marked damage 3 days ofter ischemia. These damaged neurons exhibited positive TdT dUTP nick end labeling staining. α-Fodrin breakdown product in the hippocampus increased time dependently, 2 days and 3 days after reperfusion (by 14 and 25 times for 145-, 150-kDa, and by 30-40% for 120-kDa, respectively). The amounts of Ras and phospho-Akt were significantly decreased by approximately 30-50%. The results suggest that delayed neuranal death in the hippocampal CA1 following transient forebrain ischemia is characterized by an apoptotic nature and associated with and inhibition of the Ras-Akt cascade.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University School of Medicine
Date Issued 2006
File Version Version of Record
Access Rights open access
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[isVersionOf] [URI]http://ds.cc.yamaguchi-u.ac.jp/~bulletin/index.html
Schools 医学部