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Microbial contamination of in-use ophthalmic preparations and its prevention

The bulletin of the Yamaguchi Medical School Volume 63 Issue 1-2 Page 17-24
published_at 2016
A050063000103.pdf
[fulltext] 462 KB
Title
Microbial contamination of in-use ophthalmic preparations and its prevention
Abstract
Aims: To clarify the usefulness of preservative-free ophthalmic preparations equipped with a filter. Methods: A total of 1,615 samples of in-use ophthalmic preparations were examined for microbial contamination. Results: Of 1,094 samples of preservative-containing ophthalmic preparations, 31 (2.8%) showed microbial contamination. Of 289 samples of preservative-free ophthalmic preparations without a filter, 6 (2.1%) were contaminated, consisting of 4 (13.8%) of 29 samples of hospital preparations and 2 (0.8%) of 260 samples of commercially available new quinolone antimicrobial agents. On the other hand, the microbial contamination rate in preservative-free ophthalmic preparations equipped with a filter was 0% (0 of 232 samples).The major contaminants detected in these preservative-containing ophthalmic preparations and preservative-free ophthalmic preparations without a filter were glucose-nonfermentative Gram-negative bacilli such as Pseudomonas fluorescens, Acinetobacter spp., and P. aeruginosa, coagulase (-) staphylococci, and Candida spp. The contaminant level was 10-99 colony forming units (CFU)/mL in 37.8% (14 of 37 samples), and 10^2 - 10^6 CFU/mL in 62.2% (23 of 37 samples). Conclusions: Preservative-free ophthalmic preparations equipped with a filter not only have zero risk of the oculotoxic effects of preservatives, but are also safe in terms of their lack of microbial contamination.
Creators Saisyo Atsuyuki
Creators Oie Shigeharu
Affiliate Master Yamaguchi University
[kakenhi]15501 grid.268397.1
Creators Kimura Kazuhiro
Creators Sonoda Koh-Hei
Creators Furukawa Hiroyuki
Source Identifiers [PISSN] 0513-1812 [NCID] AA00594272
Creator Keywords
ophthalmic preparation multiple-dose preservative filter microbial contamination
Resource Type departmental bulletin paper
Publishers Yamaguchi University School of Medicine
Date Issued 2016
File Version Version of Record
Access Rights open access
Schools 医学部附属病院 大学院医学系研究科(医学)