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A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita

FEMS microbiology letters Volume 212 Issue 2 Page 171-175
published_at 2002-07
Title
A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita
Creators Yokoyama Kazuhira
Creators Tateishi Takahiro
Creators Marumoto Takuya
Creators Saito Masanori
Creator Keywords
diagnostic sequence gigaspora margarita isolate-specific identification oligonucleotide probe
To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnostic sequence from both DNA preparations of single spores and colonized roots. This protocol enabled us to distinguish the isolate among several isolates from Japan, Nepal and the USA.
Subjects
農学 ( Other)
Languages eng
Resource Type journal article
Publishers Federation of European Microbiological Societies
Date Issued 2002-07
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[NCID]AA00193861
https://doi.org/10.1111/j.1574-6968.2002.tb11262.x
[isVersionOf] [URI]http://www.blackwell-synergy.com/loi/fml
Schools 農学部