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In vivo real-time monitoring and evaluation for superoxide anion radical generation with an electrochemical sensor

The bulletin of the Yamaguchi Medical School Volume 59 Issue 3-4 Page 31-34
published_at 2012
A050059000302.pdf
[fulltext] 511 KB
Title
In vivo real-time monitoring and evaluation for superoxide anion radical generation with an electrochemical sensor
Creators Fujita Motoki
Creators Maekawa Tsuyoshi
Affiliate Master Yamaguchi University
[kakenhi]15501 grid.268397.1
Creators Todani Masaki
Creators Tsuruta Ryosuke
Source Identifiers [PISSN] 0513-1812 [NCID] AA00594272
Creator Keywords
superoxide anion radical electrochemical sensor endotoxemia cerebral ischemia-reperfusion heatstroke
Recently, we established an in vivo method to directly and continuously monitor and evaluate {O_2}^{-.} using an electrochemical {O_2}^{-.} sensor. The generated {O_2}^{-.} is measured as a current and evaluated as a difference in the current from the baseline to the actual reacted {O_2}^{-.} current (ΔI) and a quantified partial value of electricity (Q), which is calculated by integration of differences between baseline and actual reacted current. To clarify the dynamics of {O_2}^{-.} in vivo and their {O_2}^{-.}-related pathophysiology, the accuracy and efficacy of this method were confirmed in phosphate-buffered saline and human blood and we applied this sensor to rat models of endotoxemia, forebrain ischemia-reperfusion (FBI/R), and heatstroke. This is a novel method for measuring {O_2}^{-.} in vivo, and could be used to monitor and treat the pathophysiology caused by excessive {O_2}^{-.} generation in animals and humans.
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University School of Medicine
Date Issued 2012
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 大学院医学系研究科(医学) 医学部附属病院