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Purification of Aconitase

The bulletin of the Yamaguchi Medical School Volume 22 Issue 2 Page 239-252
published_at 1975-06
A020022000206.pdf
[fulltext] 3.41 MB
Title
Purification of Aconitase
Creators Ogata Hatanori
Creators Nakamura Shojiro
Source Identifiers
The aconitase activity staining method on the polyacrylamide gel disc electrophoresis was devised and applied to the detection of aconitase. Aconitase of swine heart was purified by ammonium sulfate fractionation and column chromatographies. By chromatography on hydroxy-lapatite or by polyacrylamide gel electrophoresis, the ammoniun sulfate fraction of swine heart aconitase was separated into two forms. The elution volumes of both aconitase fractions were equal by column chromatography on Sephadex G-200. One form of aconitase of ammonium sulfate fraction was obtained by column chromatography on DEAE-Sephadex A-50. This form was rechromatographed on CM-Sephadex C-50 and was found to be fairly homogeneous by disc electrophoresis.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1975-06
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部