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Immobilization of Glucoamylase with Use of Photo-crosslinkable Resin Prepolymer Containing Poly (N-vinylpyrrolidone)

Memoirs of the Faculty of Engineering, Yamaguchi University Volume 33 Issue 1 Page 55-63
published_at 1982
KJ00000156438.pdf
[fulltext] 655 KB
Title
ポリビニルピロリドンをマトリックスとした光硬化性樹脂による酵素の固定化
Immobilization of Glucoamylase with Use of Photo-crosslinkable Resin Prepolymer Containing Poly (N-vinylpyrrolidone)
Abstract
A novel convenient method for entrapping enzymes or microbial cells by the use of photo-crosslinkable resin prepolymer containing poly (N-vinylpyrrolidone) and ethyleneglycol dimethacrylate was described. Glucoamylase, used as test enzyme, was entrapped successfully and easily in the resin formed by the illumination with a Hg lamp. The relative activity of the glucoamylase entrapped in the resin was affected by the proportion of enzyme and prepolymers in the immobilization system. The entrapped glucoamylase showed about 4.5 times higher K_m value and about 2.5 times lower V_<max> value compared with the native glucoamylase (pH 4.5,35℃, substrate : maltose). The pH and temperature dependencies of the enzyme reaction were also compared between the entrapped and native glucoamylases.
Creators Fukunaga Kimitoshi
Affiliate Master Yamaguchi University
[kakenhi]15501 grid.268397.1
Creators Yamashita Makoto
Creators Kimura Makoto
Source Identifiers
Subjects
材料工学 ( Other)
Languages jpn
Resource Type departmental bulletin paper
Publishers 山口大学工学部
Date Issued 1982
File Version Version of Record
Access Rights open access
Relations
[ISSN]0372-7661
[NCID]AN00244228
Schools 工学部