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A Rapid and Simple Ultramicromethod for the Estimation of Serum Pseudocholinesterase with Butyrylthiocholine as Substrate

The bulletin of the Yamaguchi Medical School Volume 15 Issue 4 Page 257-268
published_at 1968-12
A020015000401.pdf
[fulltext] 953 KB
Title
A Rapid and Simple Ultramicromethod for the Estimation of Serum Pseudocholinesterase with Butyrylthiocholine as Substrate
Creators Nakashima Koji
Creators Sera Yoko
Creators Nakano Kazuko
Source Identifiers
 A simple and rapid ultramicromethod for the determination of serum pseudocholinesterase with butyrylthiocholine as substrate was devised. Its principle consists in reading the initial velocity of the reaction of DTNB with thiocholine which is enzymatically released from butyrylthiocholine at 37 ℃. The production rate of the chromogenic substance (5-mercapto-2-nitrobenzoic acid), namely the time (in seconds) necessary for causing an increment of 0.10 in absorbance is measured at 410 mμ, in a photoelectric colorimeter equipped with a cuvette holder and a drain system warmed at constant temperature (37℃). The method is suitable not only routine clinical chemistry but also for emergency clinic. It requires minimal equipment, specimens, pipetting and time, but has satisfactory precision and accuracy.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1968-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部