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The Production of Hemagglutinin of Jananese B Enceohalitis Virus in Tissue Cultures

Bulletin of the Faculty of Agriculture, Yamaguchi University Volume 26 Page 147-160
published_at 1975
Title
組織培養における日本脳炎ウイルスの血球凝集抗原の発生
The Production of Hemagglutinin of Jananese B Enceohalitis Virus in Tissue Cultures
Creators Mannami Saburo
Creators Toda Mitsuyoshi
Creators Kanoe Masamitsu
Creators Sasaki Koji
Creators Izuchi Yuji
Source Identifiers
Cytopathic effects (CPE) of hamster-Kidney, chick-embryo and porcine-kidney cell cultures (HKTC, CETC, PKTC), hemagglutnatiion tests (HA) on the culture media, 50% Lethal dosis (LD_<50>) with mice, and hemadsorption tests (HAD) on the cultured cells were successively investigated. The results obtained were ad follows: (1) After 72hrs of virus inoculation, CPE were observed on HKTC and PKTC. A significant change was appeared on CETC. (2) The production of hemagglutinin an the result of LD_<50> were nearly correlated. The maximum titers (1:160) of HA were obtained from the media, elapsed from 72 to 96 hrs after virus inoculation, while the values of LD_<50> of the same periods were found at levels 6.4 to 7.5. (3) Before the appearances of hemagglutinin and the values of LD_<50>, hemadsorption were observed in each of cell cultures. The maximum titers of HAD were obtained from the cells, elapsed from 24 to 48 hrs efter virus inoculation. (Received Jun 30, 1975)
Subjects
農学 ( Other)
Languages jpn
Resource Type departmental bulletin paper
Publishers 山口大学農学部
Date Issued 1975
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1715
[NCID]AN00244250
Schools 農学部