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Studies on the Ribonuclease in Posterior Silkglands of Silkworm Larvae, Bombyx mori. : 2. Specincity of the Ribonuclease

Bulletin of the Faculty of Agriculture, Yamaguchi University Volume 16 Page 667-678
published_at 1965
Title
家蚕後部絹糸腺のリボヌクレアーゼに関する研究 : 第Ⅱ報酵素の特異性
Studies on the Ribonuclease in Posterior Silkglands of Silkworm Larvae, Bombyx mori. : 2. Specincity of the Ribonuclease
Creators Numa Sinji
Creators Mukai Jun-Itiro
Creators Akune Satoru
Source Identifiers
One of the two ribonucleases detected in posterior silkglands of silkworm was purified by using of ammonium sulfate fractionation, dialysis, carboxymethyl cellulose column chromatography and Sephadex G-100 gel filtration. The specificity was determined on the completely and incompletely digested yeast RNA by paper chromatography and column chromatography. This enzyme splits C'_5-phosphate bond of 3', 5'-phosphodiester linkages of ribonucleic acid. 2'-and 3'-Adenylic acid, guanylic acid, 3'-(and, or 2'-) cytidylie acid and uridylic acid (perhaps 3'-and, or 2'-) were formed, with cyclic mononucleotides as intermediate priduct.
Subjects
農学 ( Other)
Languages jpn
Resource Type departmental bulletin paper
Publishers 山口大学農学部
Date Issued 1965
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1715
[NCID]AN00244250
Schools 農学部