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Studies on the Ribonuclease in Posterior Silkglands of Silkworm Larvae, Bombyx mori. : I. Purification and General Properties of the Ribonuclease

Bulletin of the Faculty of Agriculture, Yamaguchi University Volume 16 Page 651-666
published_at 1965
Title
家蚕後部絹糸腺のリボヌクレアーゼに関する研究 : 第1報 酵素の精製と一般的性質
Studies on the Ribonuclease in Posterior Silkglands of Silkworm Larvae, Bombyx mori. : I. Purification and General Properties of the Ribonuclease
Creators Numa Sinji
Creators Mukai Jun-Itiro
Creators Akune Satoru
Source Identifiers
Two ribonuclcase active fractions (peak I and peak II) were separated from ammonium sulfate fraction of 0.3 M NaCl extract of posterior silk glands of silkworm by carboxymethy cellulose column chromatography. Peak I and peak II were purified further by carboxymethyl sephadex C-50 column chromatography and by Sephadex G-100 gel filtration, respectively. Purification degree of these two fractions were about 540 and 400 fold as compared with the original, and their recoveries were 2.4 and 2.7 %, respectiveiy. The contamination of phosphomonoesterase and phosphodiesterase w4ere not more than trace. Peak I and peak II were acid ribonuclease. These enzymes had an optimum pH at 5.3 and were not thermostavle. They were inhibited by Mn^<2+>, Ca^<2+> and Na-phosphate and activated by mg^<2+> and Na^+. Further more peak II was inhibited by Zn^<2+>.
Subjects
農学 ( Other)
Languages jpn
Resource Type departmental bulletin paper
Publishers 山口大学農学部
Date Issued 1965
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1715
[NCID]AN00244250
Schools 農学部