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The Influence of Chemotherapy on Proliferating Cell Nuclear Antigen of Glioblastoma Cell Line

山口医学 Volume 43 Issue 1 Page 79-86
published_at 1994-02
Title
多形性膠芽腫T98G細胞における化学療法のProliferating cell nuclear antigenに及ぼす影響について : ranimustineとetoposideを用いた検討
The Influence of Chemotherapy on Proliferating Cell Nuclear Antigen of Glioblastoma Cell Line
Creators Furutani Yasuhiro
Source Identifiers
Creator Keywords
glioblastoma cell line chemotherapy proliferating cell nuclear antigen
In this study we analized cell kinetics of glioblastoma cell line (T98G) under growth suppresive state by use of PCNA-DNA and BrdU-DNA simultaneous double stain method (flow cytometry). The growth suppresive state was induced by two kinds of anticancer agents of MCNU and etoposide. In spite of decrease of BrdU antigen by both anticancer agents, PCNA antigen and PCNA positive ratio increased compared with that of control in the early stage of chemotherapy. Especially PCNA antigen was increased more than two times of control by the proliferation suppresive concentration of etoposide that acted after consumption of PCNA in the series of DNA synthesis. We considered that was caused by change of cell cycle, negative feedback against growth suppresive state and suppression of comsumption of PCNA. It can be summarized that PCNA antigen and PCNA positive ratio under growth suppresive state by anticancer agent don't reflect truly tumor proliferation in the early stage of chemotherapy, and we can't judge the effect of anticancer agent by analysis of cell kinetics used PCNA. In the future, compared with control growth suppresive state would be caught in early stage of chemotherapy by increase of PCNA antigen.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1994-02
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部