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A Method of Cultivation and Multiplication of Japanese B Encephalitis virus in the Deembryonated Eggs. : 3.On the Experiment using the Various Salt Solution as Medium and Histo-pathological Observation

Bulletin of the Faculty of Agriculture, Yamaguchi University Volume 13 Page 307-322
published_at 1962
Title
日本脳炎ウイルスの卵殻を用いての培養並びに増殖法 : 第III報 各種塩類溶液を培養液とした諸実験並びに病理組織学的所見
A Method of Cultivation and Multiplication of Japanese B Encephalitis virus in the Deembryonated Eggs. : 3.On the Experiment using the Various Salt Solution as Medium and Histo-pathological Observation
Creators Toda Mitsuyoshi
Source Identifiers
According to the methods which were used in the former experiments, every kind of salt solution, for example, PBS, Tyrode, Hanks, Earle and LYH (added with Lactalbumin Hydrolysate in the rate of 0.5% and with yeast Extract in the rate of 0.01% into Hanks solution respectively) and EYL (added with the same ingredients in the same rate as was LYH) was used as medium and the grade of multiplication of virus was measured by LD_<50> of the infected mouse in the same way as the former experiments. Furthermore, the histo-pathological examination was concurrently carried out on the chorio allantoic membrane of these materials to find the morphological changes of membrane occurred with the virus multiplication. Some lesurts were obtained in these experiments. The results were as follows: 1. Among the various salt solutions used in the present expriment LYH and EYL was the best for the virus multiplication and gave the almost fixed virus titer, but it was risen up agein by adding with ox serum in the rate of 10%. ....
Subjects
農学 ( Other)
Languages jpn
Resource Type departmental bulletin paper
Publishers 山口大学農学部
Date Issued 1962
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1715
[NCID]AN00244250
Schools 農学部