Studies on the Enzymatic Synthesis of Thiamine by Staphylococcus Aureus

Studies on the Enzymatic Synthesis of Thiamine by Staphylococcus Aureus

Creators Tsubota Yoko

Creators Hayashi Ryoji

Studies on the enzymatic synthesis of thiamine by Staphylococcus aureus FDA 209-P were carried out and following results were obtained. 1) The active form of pyrimidine was OMP-PP. 2) Free Th as well as its phosphorylated derivatives was sctive as a substrate for the acetone-dried powder of the organisms and the crude enzyme preparation extracted from the powder. 3) Th-P was the most active form of Th for the enzyme preparation with had been purified by the treatment with ammonium sulfate and chromatography on DEAE-cellulose. The activity of free Th for the partially purefied enzyme was negligible. 4) The reaction products from OMP-PP and Th-P of the purified enzyme preparation were TDP and TMP as examined by paper chromatographic and bioautographic method. 5) The activity of the enzyme was increased by the addition of Mn¨ Mg¨or Cd¨. 6) The rhiamine-synthesizing enzyme was comparatively thermostable.

[ISSN]0513-1812

[NCID]AA00594272