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Studies on the Enzymatic Synthesis of Thiamine by Staphylococcus Aureus

The bulletin of the Yamaguchi Medical School Volume 10 Issue 4 Page 215-225
published_at 1963-12
A020010000403.pdf
[fulltext] 630 KB
Title
Studies on the Enzymatic Synthesis of Thiamine by Staphylococcus Aureus
Creators Tsubota Yoko
Creators Hayashi Ryoji
Source Identifiers
Studies on the enzymatic synthesis of thiamine by Staphylococcus aureus FDA 209-P were carried out and following results were obtained. 1) The active form of pyrimidine was OMP-PP. 2) Free Th as well as its phosphorylated derivatives was sctive as a substrate for the acetone-dried powder of the organisms and the crude enzyme preparation extracted from the powder. 3) Th-P was the most active form of Th for the enzyme preparation with had been purified by the treatment with ammonium sulfate and chromatography on DEAE-cellulose. The activity of free Th for the partially purefied enzyme was negligible. 4) The reaction products from OMP-PP and Th-P of the purified enzyme preparation were TDP and TMP as examined by paper chromatographic and bioautographic method. 5) The activity of the enzyme was increased by the addition of Mn¨ Mg¨or Cd¨. 6) The rhiamine-synthesizing enzyme was comparatively thermostable.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1963-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部