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Artefacts Appearing at the Crossing Pointson the Lines of Reactants in the Crossing Electrophoresis

The bulletin of the Yamaguchi Medical School Volume 11 Issue 2 Page 63-76
published_at 1964-06
A020011000202.pdf
[fulltext] 4.83 MB
Title
Artefacts Appearing at the Crossing Pointson the Lines of Reactants in the Crossing Electrophoresis
Creators Suzuno Ryosuke
Creators Tanabe Yoshio
Creators Nakamura Shojiro
Source Identifiers
When one substance is applied on a line parallel to the electrical field and the other substance on a line perpendicular to it, the line of the latter may be grooved at the crossing point by the electrophoresis, if they reacted each other. Thus the formation of the groove can be used as a criterion to detect whether two reactants react or not. (Principle of crossing electrophoresis.) Artificial groovings in the line of a substance were theoretically predicted to be duw to hugh viscosity, high ionic strength, and/or large difference in pH of the solution of the other reactant from those of the buffer solution used for wlwctrophoresis. Artefacts due to mechanical damage of the filter paper and those dueto adsorption of protein were also shown to occur. Discussions were presented on the prerequisites for avoiding the formation of artificial grooves in the line of reactant, due to the above causes, and the use of appropriate contrals was recommended.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1964-06
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部