Heat Denaturation Test for Unstable Hemoglobin, with a Note on Its Application to Hemoglobin Survey in Japan

Heat Denaturation Test for Unstable Hemoglobin, with a Note on Its Application to Hemoglobin Survey in Japan

Creators Ueda Satoshi

Creators Matsuoka Miyoko

Creators Miyaji Takaoki

Creators Ueda Naoki

Creators Aoki Kenzo

Creators Izawa Michi

Creators Shibata Susumu

　Dacie's heat denaturation test was modified in order to get a practical procedure for detecting unstable hemoglobin in hemolysates. For the purpose of screening a centrifugation method was devised : hemolysate, phosphate buffer solution (0.1 M, pH 7.4), and zinc chloride solution were mixed in equal proportions (0.2 ml each) in a test tube and transferred to a capillary tube (0.3 × 12 cm). The capillary tube was sealed at one end by fusion in a flame of a burner so that the mixture might be contained safely in it, warmed at 50℃ for 60 minutes, and then centrifuged at 3000 rpm (1700 G) for 5 minutes to read the amount of precipitate sedimented at the bottom in terms of the percentage of the total co1umn of the mixture. A reading larger than 3.5 per cent is suggesive of the presence of unstable hemoglobin in hemolysate. Such a hemolysate should be examined by spectrophotometric method to connrm the result. Namely, hemolysate (30 μl) was mixed with warm (55℃) phosphate buffer solution (0.1 M, pH 7.4

[ISSN]0513-1812

[NCID]AA00594272