Studies on the Technical Procedures in Scanning Electron Microscopy : 2. Scanning Electron Microscopic Method for Spirality of Short-type Bacteria, with Special Reference to Preparation

走査電子顕微鏡実験法術技の研究 : 第2報 短い細菌細胞の旋転性の研究法,特に試料準備について

Studies on the Technical Procedures in Scanning Electron Microscopy : 2. Scanning Electron Microscopic Method for Spirality of Short-type Bacteria, with Special Reference to Preparation

Creators Konishi Hisanori

Creators Yoshii Zensaku

Creators Kamata Mizuho

Creators Nakano Hiroyuki

Creators Okada Masahiro

Creators Katayama Atsushi

Creators Nakao Toshiki

Creators Sugiyama Kenji

Creators Mizuno Akira

Creators Mii Hirofumi

Creators Akitomi Hisaji

Creators Ito Takeo

Creators Kegoya Yomiko

In order to investigate a twisting body of the short-type bacteria, the elongation method using Cephalexin was attempted in scanning electron microscopy. Two species of Spirillum and two species of vibrio were used, and Spirillum itersonii with definite spiral was also used for the positive control and Escherichia coli without spiral for the negative control, respectively. Two bacteria for controls preserved their shapes with or without spirality in the media containing Cephalexin. Other spirilla and vibrio provided the elongated cell bodies around concentration of MIC and demonstrated definite spiral-direction. Therefore, this method was considered to be useful for investigation of the twisted body of the short-type becteria.

[ISSN]0513-1731

[NCID]AN00243156