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Kadokawa Hiroya

Affiliate Master Yamaguchi University

Alpha-synuclein expression in oxytocin neurons of young and old bovine brains

Journal of Reproduction and Development Page 2021-126-
published_at 2024
JRD-2024-020_POSTedited.pdf
[fulltext] 1.74 MB
Title
Alpha-synuclein expression in oxytocin neurons of young and old bovine brains
Abstract
Understanding of central nervous system mechanisms underlying age-related infertility remains limited. Fibril α-synuclein, distinct from its monomeric form, is implicated in age-related diseases. Notably, fibril α-synuclein spreads among neurons, similar to prions, from damaged old neurons in cortex and hippocampus to healthy neurons. However, less is known whether α-synuclein propagates into oxytocin neurons, which play crucial roles in reproduction. We compared α-synuclein expression in the oxytocin neurons in suprachiasmatic nucleus (SCN), supraoptic nucleus (SON), paraventricular hypothalamic nucleus (PVN), and posterior pituitary (PP) gland of healthy heifers and aged cows to determine its role in age-related infertility. We analyzed mRNA and protein expression, along with Congo red histochemistry and fluorescent immunohistochemistry for oxytocin and α-synuclein, followed by confocal microscopy with Congo red staining. Both mRNA and protein expressions of α-synuclein were confirmed in the bovine cortex, hippocampus, SCN, SON, PVN, and PP tissues. Significant differences in α-synuclein mRNA expressions were observed in the cortex and hippocampus between young heifers and old cows. Western blots showed five bands of α-synuclein, probably reflecting monomers, dimers, and oligomers, in the cortex, hippocampus, SCN, SON, PVN, and PP tissues, and there were significant differences in some bands between the young heifers and old cows. Bright-field and polarized light microscopy did not detect obvious amyloid deposition in the aged hypothalami; however, higher-sensitive confocal microscopy unveiled strong positive signals for Congo red and α-synuclein in oxytocin neurons in the aged hypothalami. α-synuclein was expressed in oxytocin neurons, and some differences were observed between young and old hypothalami.
Creators Kadokawa Hiroya
[e_rad]80370592 80370592
Affiliate Master Yamaguchi University
[kakenhi]15501 grid.268397.1
Source Identifiers [PISSN] 0916-8818 [EISSN] 1348-4400 [NCID] AA10936678 [NCID] AA12018734
Creator Keywords
Ageing Paraventricular hypothalamic nucleus Posterior pituitary gland Suprachiasmatic nucleus Supraoptic nucleus
Languages eng
Resource Type journal article
Publishers The Society for Reproduction and Development
Date Issued 2024
Rights
Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license(https://creativecommons.org/licenses/by-nc-nd/4.0/)
Copyright: 日本繁殖生物学会(The Japanese Society of Animal Reproduction)()
File Version Accepted Manuscript
Access Rights open access
Relations
[isVersionOf] https://doi.org/10.1262/jrd.2024-020
Funding Refs
Japan Society for the Promotion of Science [crossref_funder]https://doi.org/10.13039/501100001691
Award ウシ視床下部で発見された泌乳・加齢による繁殖障害の原因物質:現場での応用に向けて 21H02345
Schools 共同獣医学部