A method detecting bacteria in culture medium by simultaneous measurement of electrical impedance and turbidity

Creators Hashimoto Hajime

Creators Miike Hidetoshi

Creators Ebina Yoshio

Creators Miyaji Takaoki

Simultaneous measurement of impedance and turbidity changes in culture medium was proposed. The impedance change was measured by means of a four-electrode method. Two characteristic parameters for Escherichia coli and Staphylococcus epidermidis grown in Brain Heart Infusion broth were obtained. The growth speed α (inverse of generation time) and the delay time T of impedance change were computed as characteristic parameters from measured quantities. Since bacteria had inherent α and T, they were tentatively represented in term of a characteristic vector with two components α and T. Two bacteria were clearly discriminated on the two dimensional space.

[ISSN]0386-3433

[NCID]AA0086073X