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Maekawa Tsuyoshi

Affiliate Master Yamaguchi University

Xanthine oxidase is one of the major sources of superoxide anion radicals in blood after reperfusion in rats with forebrain ischemia/reperfusion

Brain research : international multidisciplinary journal devoted to fundamental research in the brain sciences Volume 1305 Page 158-167
published_at 2009-12-11
2010010091.pdf
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Title
Xanthine oxidase is one of the major sources of superoxide anion radicals in blood after reperfusion in rats with forebrain ischemia/reperfusion
Creators Ono Takeru
Creators Tsuruta Ryosuke
Creators Fujita Motoki
Creators Shinagawa Aki Hiromi
Creators Kutsuna Satoshi
Creators Kawamura Yoshikatsu
Creators Wakatsuki Jun
Creators Aoki Tetsuya
Creators Kobayashi Chihiro
Creators Kasaoka Shunji
Creators Maruyama Ikuro
Creators Yuasa Makoto
Creators Maekawa Tsuyoshi
Creator Keywords
allopurinol electrochemical sensor forebrain ischemia reperfusion superoxide anion radical xanthine oxidase inhibitor
We recently reported that excessive superoxide anion radical (O_2–•) was generated in the jugular vein during reperfusion in rats with forebrain ischemia-reperfusion using a novel electrochemical sensor and excessive O_2–• generation was associated with oxidative stress, early inflammation, and endothelial injury. However, the source of O_2–• was still unclear. Therefore, we used allopurinol, a potent inhibitor of xanthine oxidase (XO), to clarify the source of O_2–• generated in rats with forebrain ischemia-reperfusion. The increased O_2–• current and the quantified partial value of electricity (Q), which was calculated by the integration of the current, were significantly attenuated after reperfusion by pretreatment with allopurinol. Malondialdehyde (MDA) in the brain and plasma, High-mobility group box 1 (HMGB1) in plasma, and intercellular adhesion molecule-1 (ICAM-1) in the brain and plasma were significantly attenuated in rats pretreated with allopurinol with dose-dependency in comparison to those in control rats. There were significant correlations between total Q and MDA, HMGB, or ICAM-1 in the brain and plasma. Allopurinol pretreatment suppressed O_2–• generation in the brain-perfused blood in the jugular vein, and oxidative stress, early inflammation, and endothelial injury in the acute phase of forebrain ischemia-reperfusion. Thus, XO is one of the major sources of O_2–• in blood after reperfusion in rats with forebrain ischemia-reperfusion.
Languages eng
Resource Type journal article
Publishers Elsevier
Date Issued 2009-12-11
Rights
Copyright c2009 Elsevier Inc. All rights reserved.()
File Version Author’s Original
Access Rights open access
Relations
[ISSN]0006-8993
[NCID]AA0057324X
[PMID]info:pmid/19781528
info:doi/10.1016/j.brainres.2009.09.061
[isVersionOf] [URI]http://www.sciencedirect.com/science/journal/00068993
Schools 医学部附属病院 大学院医学系研究科(医学)