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Yamauchi Naoki

Affiliate Master Yamaguchi University

Production and characterization of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of Japanese bunching onion (A. fistulosum L.)

Genes & genetic systems Volume 79 Issue 5 Page 263-269
published_at 2004
2009010007.pdf
[fulltext] 1.41 MB
Title
Production and characterization of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of Japanese bunching onion (A. fistulosum L.)
Creators Hang Tran Thi Minh
Creators Shigyo Masayoshi
Creators Yamauchi Naoki
Creators Tashiro Yosuke
Creator Keywords
alien addition line Allium cepa Allium fistulosum flow cytometry isozyme
First and second backcrosses of amphidiploid hybrids (2n = 4x = 32, genomes AAFF) between shallot (Allium cepa Aggregatum group) and A. fistulosum were conducted to produce A. cepa - A. fistulosum alien addition lines. When shallot (A. cepa Aggregatum group) was used as a pollinator, the amphidiploids and allotriploids set germinable BC1 and BC2 seeds, respectively. The 237 BC1 plants mainly consisted of 170 allotriploids (2n = 3x = 24, AAF) and 42 hypo-allotriploids possessing 23 chromosomes, i.e., single-alien deletions (2n = 3x-1 = 23, AAF-nF). The single-alien deletions in the BC1 progeny showed dwarfing characteristics and were discriminated from the allotriploids (2n = 24) and hyper-allotriploids (2n = 25) by means of flow cytometric analysis. The chromosome numbers of 46 BC2 seedlings varied from 16 to 24. Eight monosomic additions (2n = 2x+1 = 17, AA+nF) and 20 single-alien deletions were found in these BC2 seedlings. Consequently, six kinds of A. cepa - A. fistulosum alien chromosome additions possessing different chromosome numbers (2n = 17, 18, 20, 21, 22, 23) were recognized in the BC1 and BC2 populations. A total of 79 aneuploids, including 62 single-alien deletions, were analyzed by a chromosome 6F-specific isozyme marker (Got-2) in order to recognize its existence in their chromosome complements. This analysis revealed that two out of 62 single-alien deletions did not possess 6F. One (AAF-6F) out of the possible eight single-alien deletions could be identified at first. The present study is a first step toward the development of a useful tool, such as a complete set of eight different single-alien deletions, for the rapid chromosomal assignment of genes and genetic markers in A. fistulosum.
Languages eng
Resource Type journal article
Publishers 日本遺伝学会
Date Issued 2004
File Version Version of Record
Access Rights open access
Relations
[ISSN]1341-7568
[NCID]AA11077421
https://doi.org/10.1266/ggs.79.263
[isVersionOf] [URI]http://www.jstage.jst.go.jp/browse/ggs/_vols
Schools 農学部