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Yokoyama Kazuhira

Affiliate Master Yamaguchi University

Estimation of mycorrhizal colonization of the roots of oak seedlings inoculated with an ectomycorrhizal fungus, Laccaria amethystea

Soil science and plant nutrition Volume 49 Issue 4 Page 641-645
published_at 2003-08
2007020024.pdf
[fulltext] 2.93 MB
Title
Estimation of mycorrhizal colonization of the roots of oak seedlings inoculated with an ectomycorrhizal fungus, Laccaria amethystea
Creators Tateishi Takahiro
Creators Yokoyama Kazuhira
Creators Kohno Nobuyuki
Creators Okabe Hiroaki
Creators Marumoto Takuya
Creator Keywords
colonization level ectomycorrhizal fungi Laccaria amethystea oak seedlings root tips
Six-month-old seedlings of Quercus serrata and Quercus glauca in a nursery were inoculated with the ectomycorrhizal fungus Laccaria amethystea encapsulated in alginate gel and grown in the nursery. The seedlings were collected at 1, 3, and 5 months after the inoculation and examined for colonization of the root system with ectomycorrhizal fungi. The roots within 5 months after the inoculation showed rudimentary ectomycorrhizal colonization. The level of colonization of the root system was estimated based on the intensity of hyphal covering on the root tips by staining with a fluorescent dye and expressed as an index of mycorrhizal colonization (IMC). IMC increased with the time after inoculation and reached values of 4 and 12% in Q. serrata and Q. glauca, respectively at 5 months after the inoculation. The determination of IMC may enable to assess the development of mycorrhizal colonization of the root system that shows rudimentary ectomycorrhizas after the inoculation.
Subjects
農学 ( Other)
Languages eng
Resource Type journal article
Publishers 日本土壌肥料学会
Date Issued 2003-08
File Version Version of Record
Access Rights open access
Relations
[ISSN]0038-0768
[NCID]AA00844314
[isVersionOf] [NAID]http://ci.nii.ac.jp/naid/10012149035/
[isVersionOf] [URI]http://www.blackwellpublishing.com/journal.asp?ref=0038-0768&site=1
Schools 農学部