Yokoyama Kazuhira
Affiliate Master
Yamaguchi University
A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita
FEMS microbiology letters Volume 212 Issue 2
Page 171-175
published_at 2002-07
Title
A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita
Creators
Tateishi Takahiro
Creators
Saito Masanori
Creator Keywords
diagnostic sequence
gigaspora margarita
isolate-specific identification
oligonucleotide probe
To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnostic sequence from both DNA preparations of single spores and colonized roots. This protocol enabled us to distinguish the isolate among several isolates from Japan, Nepal and the USA.
Languages
eng
Resource Type
journal article
Publishers
Federation of European Microbiological Societies
Date Issued
2002-07
File Version
Not Applicable (or Unknown)
Access Rights
metadata only access
Relations
[NCID]AA00193861
[isVersionOf]
[URI]http://www.blackwell-synergy.com/loi/fml
Schools
農学部