Introduction of biomarker into Ralstonia solanacearum for dynamics analysis in soil

ナス科植物青枯病菌(Ralstonia solanacearum)の土壌中での動態解析を目的とした生態マーカーの導入

Introduction of biomarker into Ralstonia solanacearum for dynamics analysis in soil

Creators Idukuhara Miho

Creators Sakai Masao

Creators Takaki Shigeki

Creators Yokoyama Kazuhira

Creators Marumoto Takuya

A biomarker, gfp gene, was introduced into a strain of Ralstonia solanacearum isolated from tomato to investigate the fate of this strain in soil. Tn5-mutants of the strain SL8 ware virulent against tomatoes. The behavior of these gfp-fused mutants was similar to that of the wild type strain both in the culture medium and in soil. In the case of the gfp-fused mutants in which the production of extracellular polysaccharides (EPS) was impaired or absent (EPS-), the fluorescence of GFP was useful to discriminate between the inoculated strains from other indigenous bacteria in soil. In conclusin, the biomarker GFP allowed the count of both wild type strains, one which produced large amounts of EPS and the other PC-type mutants in terms of EPS. Subsequently, the gfp-fused mutants of Ralstonia solanacearum enabled to analyze the factors that induce phenotypic conversion from wild type strains under environmental conditions.

[ISSN]0912-2184

[NCID]AN00352796