Relationship between The Expression of DNA Repair Enzyme : ERCC1 (excision repair cross-complementing l) and Resistance to CDDP (cis-dichlorodiammineplatinum(II) ) in Human Esophageal Squamous Cell Carcinoma Cell Lines.
山口医学 Volume 46 Issue 4
Page 211-222
published_at 1997-08
Title
ヒト食道癌細胞株を用いたDNA修復酵素ERCC1(excision repair cross-complementing 1)の発現とシスプラチン感受性の検討
Relationship between The Expression of DNA Repair Enzyme : ERCC1 (excision repair cross-complementing l) and Resistance to CDDP (cis-dichlorodiammineplatinum(II) ) in Human Esophageal Squamous Cell Carcinoma Cell Lines.
Creators
Abe Toshihiro
Source Identifiers
Creator Keywords
ERCC1 (excision repair cross-complementing 1)
DNA修復
シスプラチン
薬剤耐性
食道癌
Chemotherapy using CDDP has demonstrated efficacy in esophageal cancer. The activity of ERCC1 is well correlated with resistance to CDDP in ovarian cancer and hematopoietic malignancies. Studies in vitro were therefore undertaken to investigate the relationship between the expression of ERCC1 and resistance to CDDP in six human esophageal cancer cell lines (ECC) (YES-1, -2, -3, -4, -5, _6) using RT-PCR, Western blot analysis and immunostaining for ERCC1 expression and MTT assay or sensitivity against CDDP. IC_50 of CDDP in YES-1, -2, -3, -4, -5, _6 cells were > 20, 5.0 ± 0,80, 6.0 ± 0.50, 0.65 ± 0.14, > 20, and > 20 ng/ml, respectively. YES-4 cells showed high sensitivity to CDDP compared with 5 other ECCs. The expression of ERCC1 mRNA and its protein in YES-4 and -5 significantly decreased compared with 4 other ECCs. The sensitivity for CDDP in ECCs were correlated with the ERCC1 expression, except for YES-5. These results suggest that ERCC1 may contribute to resistance to CDDP in esophageal cancer.
Languages
jpn
Resource Type
journal article
Publishers
山口大学医学会
Date Issued
1997-08
File Version
Not Applicable (or Unknown)
Access Rights
metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools
医学部