Analysis of Squamous Cell Carcinoma Using Monoclonal and Polyclonal Antibodies against Cell Surface Antigens

細胞表面抗原に対するモノクローナル抗体を用いた扁平上皮癌の解析

Analysis of Squamous Cell Carcinoma Using Monoclonal and Polyclonal Antibodies against Cell Surface Antigens

Creators Inoue Takashi

Cell surface antigens are important for cell differentiation, cell to cell interaction and as receptors for hormones and cytokines. To analyze squamous cell carcinoma, monoclonal and polyclonal antibodies against cell surface antigens were used. The antigens were detected by immunohistochemistry methods and cellular radioimmunoassay. Rats and transplantable rat squamous cell carcinoma were used. The following results were obtained: 1. To characterize further the nature of transplantable rat squamous cell carcinoma (FF6) in DA strain rats, several monoclonal antibodies were used. Cytokeratin and UB-14 antigens were detected on the well differentiated rat squamous cell carcinoma epithelium, while laminin, fibronectin and collagen were visualized among the epithelial components of FF6 tissues using immunofluorescence. 2. A monoclonal antibody against FF6 (UB-17) stained cancer cell membranes of FF6 tissues as well as the basal and spinous cell layers of normal rat stratified squamous epithelium, the staining being particularly heavy on the spinous cell layer. The antigen on squamous epithelium was detectable in day 18 fetal rat skin. UB-17 cross-reacted with human normal skin and squamous cell carcinoma. 3. To characterize the nature of UB-17 antigen, which was clearly detected on the surface of human gingival cells by immunoelectron microscopy, a comparison of tissue distribution was made between UB-17 and other known monoclonal antibodies against human squamous cell carcinomas, such as VM-2, BM2, HECD-1 and G6K12 among which G6K12 was found to be close to UB-17. The antigens recognized by VM-2 and HECD-1 were antigens related to cell adhesion. 4. UB-17 antigen was unstable in the presence of most detergents, but heat stable, and some antigenic epitopes were soluble in water. 5. Cellular radioimmunoassay was possible using either fresh or glutaraldehyde-fixed FF6 homogenate. Furthermore, the homogenate of FF6, serum, and ascites from FF6 tumor bearing rats showed significant antigenicity when used for radioactive binding inhibition assay, suggesting the possible use of the assay for serum diagnosis in the clinical field. From these results, it is suggested that transplantable rat squamous cell carcinoma (FF6) and a monoclonal antibody against it (UB-17) are useful tools for studying squamous cell carcinoma as well as for clinical use in the future.

[ISSN]0513-1731

[NCID]AN00243156