Microassay for Proteins on Nitrocellulose Filter Using Protein Dye-Staining Procedure

ニトロセルロース膜を用いたタンパク微量測定法

Microassay for Proteins on Nitrocellulose Filter Using Protein Dye-Staining Procedure

Creators Nakamura Kazuyuki

Creators Tanaka Tatehiko

Creators Fujimoto Masanori

Creators Kuwahara Akira

Creators Suzuno Ryousuke

Creators Suzuki Isao

Creators Takeo Kazusuke

A simple and rapid microassay for proteins utilizing the protein dye-staining procedure with a nitrocellulose (NC) filter is described. Proteins were directly bound to an NC filter using the ”BIO DOT” microfiltration apparatus to ensure their uniformity. The proteins were then stained with a dye, and the optical density of the stained protein spots was directly measured by a densitometer. A good linearity between the optical density and the amount of protein was obtained in the range 0.05-10μg. A larger number of samples (up to 96 samples) could be assayed within 1.5 h simultaneously. Furthermore, contaminating chemicals, such as amino acids, sugars, reducing agents, chelating agents, deoxyribonucleic acid, nucleotides, or tris (hydroxymethyl) aminomethane, did not interfere with the assay. The usefulness of the assay for the quantitation of a small amount of proteins in body fluids is discussed.

[ISSN]0513-1731

[NCID]AN00243156