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Cell Kinetics of Mouse L_5 Cells

山口医学 Volume 30 Issue 1 Page 31-36
published_at 1981-03
Title
マウスL_5細胞の増殖動態
Cell Kinetics of Mouse L_5 Cells
Creators Oku Tomozo
Creators Yamaoka Hideki
Creators Matsumoto Takafumi
Creators Okuda Shinichiro
Creators Ishiguro Kimio
Creators Takahashi Manabu
Source Identifiers
Cell kinetics of mouse L_5 cells was studied using ^<3>H-thymidine (^<3>H-TdR) autoradiography. In contrast to the conventional method of cell cycle analysis, T_G1+1/2M and Ts were estimated independently from labeling index curve after mitotic selection and after release from double thymidine block, respectively. T_G2+1/2M was obtained by the method analogous to the fraction of labeled mitoses (FLM). The results can be summerized that T_G2+1/2M=7, Ts=8.5, T_G2+1/2M=7hr. Growth fraction (GF) was 85%. This method seems advantageous over the FLM or double labeling method in which the data are illresolved due to cumulative variation of several consecutive phases.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1981-03
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部