Cell Kinetics of Mouse L_5 Cells
山口医学 Volume 30 Issue 1
Page 31-36
published_at 1981-03
Title
マウスL_5細胞の増殖動態
Cell Kinetics of Mouse L_5 Cells
Creators
Oku Tomozo
Creators
Yamaoka Hideki
Creators
Matsumoto Takafumi
Creators
Okuda Shinichiro
Creators
Ishiguro Kimio
Creators
Takahashi Manabu
Source Identifiers
Cell kinetics of mouse L_5 cells was studied using ^<3>H-thymidine (^<3>H-TdR) autoradiography. In contrast to the conventional method of cell cycle analysis, T_G1+1/2M and Ts were estimated independently from labeling index curve after mitotic selection and after release from double thymidine block, respectively. T_G2+1/2M was obtained by the method analogous to the fraction of labeled mitoses (FLM). The results can be summerized that T_G2+1/2M=7, Ts=8.5, T_G2+1/2M=7hr. Growth fraction (GF) was 85%. This method seems advantageous over the FLM or double labeling method in which the data are illresolved due to cumulative variation of several consecutive phases.
Languages
jpn
Resource Type
journal article
Publishers
山口大学医学会
Date Issued
1981-03
File Version
Not Applicable (or Unknown)
Access Rights
metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools
医学部