Contents Menu

Study on Hydrolysis gor Feulgen Microspectrophotometry

山口医学 Volume 19 Issue 1 Page 19-26
published_at 1970-03
Title
Feulgen加水分解法の検討
Study on Hydrolysis gor Feulgen Microspectrophotometry
Creators Tomonaga Susumu
Creators Teranaka Masaki
Creators Ogata Hiroaki
Creators Nomura Akito
Creators Kato Seiji
Creators awaya Kazuhiko
Source Identifiers
In the present study, examinations of Feulgen hydrolysis were carried out by the microspectrophotometric measurement of the relative DNA contents in lymphocyte nuclei. Smears of thymus of male mice, 2-month old, were made. The smears were air-dried for at least 1 hour, fixed in methanol-formalin-acetic acid mixed 85 : 10 : 5, in Carnoy's solution or in formalin vapor for 30 minutes, and subjected to the Feulgen-staining procedure. Feulgen hydrolysis was performed either in 1 N HCl at 60℃ in a constant temperature water bath or in 5 N HCl at 25℃ (±3℃). After washing, smears were stained in decolorized basic fuchsin for 3 hours, and rinsed three times with SO_2 for 15 minutes and once tap water for 5 minutes. The relative DNA content was measured in 10 to 20 small lymphocytes from each smear by microspectrophotometry at 560 mμ by the scanning procedure.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1970-03
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部