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A Comment on Calculation of Feulgen-DNA Content Determination by Microspectrophotometry

山口医学 Volume 19 Issue 1 Page 9-18
published_at 1970-03
Title
顕微分光測光法による Feulgen-DNA 定量値の算出法に関する一考察
A Comment on Calculation of Feulgen-DNA Content Determination by Microspectrophotometry
Creators Tomonaga Susumu
Creators Teranaka Masaki
Creators Ogata Hiroaki
Creators Nomura Akito
Creators Kato Seiji
Creators Awaya Kazuhiko
Source Identifiers
The relative values of DNA contents of lymphocyte nuclei were estimated by the scanning method of Feulgen microspectrophotometry. The caluculation were made by the following two formulas Q_a = ∫| x | f(x)dx (a) Q_b = r/2 ∫f(x)dx (b) where Q_a and Q_b : the relative value of DNA content f(x) : the curve obtained actually tracing the nuclear along the diameter with the spot light |x| : a distance from the center of the nuclear diameter r : the radius of the nucleus. The values were calculated from the areas surrounded by the extinction curves in the formula (b), and from the volumes of the rotating body of the extinction curves in the formula (a). The calculation from the formula (a), which was recommended by Seno and Utsumi, has been proved very useful in minimizing the distribution errors and the Schwarzschild-Villiger effect. But it is so cumbersome in its practical use that a simpler and dependable method is desirable. We have found that the formula (b) fits for this purpose and the values calculated from it is fully reliable as a result of statistical analysis.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1970-03
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部