A Comment on Calculation of Feulgen-DNA Content Determination by Microspectrophotometry
山口医学 Volume 19 Issue 1
Page 9-18
published_at 1970-03
Title
顕微分光測光法による Feulgen-DNA 定量値の算出法に関する一考察
A Comment on Calculation of Feulgen-DNA Content Determination by Microspectrophotometry
Creators
Tomonaga Susumu
Creators
Teranaka Masaki
Creators
Ogata Hiroaki
Creators
Nomura Akito
Creators
Kato Seiji
Creators
Awaya Kazuhiko
Source Identifiers
The relative values of DNA contents of lymphocyte nuclei were estimated by the scanning method of Feulgen microspectrophotometry. The caluculation were made by the following two formulas Q_a = ∫| x | f(x)dx (a) Q_b = r/2 ∫f(x)dx (b) where Q_a and Q_b : the relative value of DNA content f(x) : the curve obtained actually tracing the nuclear along the diameter with the spot light |x| : a distance from the center of the nuclear diameter r : the radius of the nucleus. The values were calculated from the areas surrounded by the extinction curves in the formula (b), and from the volumes of the rotating body of the extinction curves in the formula (a). The calculation from the formula (a), which was recommended by Seno and Utsumi, has been proved very useful in minimizing the distribution errors and the Schwarzschild-Villiger effect. But it is so cumbersome in its practical use that a simpler and dependable method is desirable. We have found that the formula (b) fits for this purpose and the values calculated from it is fully reliable as a result of statistical analysis.
Languages
jpn
Resource Type
journal article
Publishers
山口大学医学会
Date Issued
1970-03
File Version
Not Applicable (or Unknown)
Access Rights
metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools
医学部