Indirect hemagglutination test for diagnosis of canine filariasis

Indirect hemagglutination test for diagnosis of canine filariasis

Creators Hayasaki Mineo

Four kinds of Dirofilaria immitis antigens, prepared from intrauterine micro-filariae (I-Mf), circulating microfilariae (C-Mf), migrating larvae and adult worms (A-Di), were assessed by indirect hemagglutination (IHA) test in terms of immunological specificity and sensitivity. The highest specificity and sensitivity were observed in phosphate buffered saline (PBS) extract of I-Mf among these antigens. In contrast, PBS extract of C-Mf was found to have extremely low IHA antigenicity. Cross reactions between D. immitis and some intestinal parasites, such as Toxocara canis, Ancylostoma caninum and Trichuris vulpis, were evaluated by agglutinin absorption assay. It was revealed that no substantial cross reaction was present between I-Mf or A-Di and the above three intestinal parasites. Agglutinin absorption assay also suggested that the antigenicity of A-Di antigen differed from that of I-Mf antigen, although some common antigens were present between them. The present results indicate that I-Mf antigen is useful for IHA test in D. immitis infection in dogs.

[ISSN]0021-5295

[NCID]AN00191788

[isVersionOf] [URI]http://www.jstage.jst.go.jp/browse/jvms/-char/ja

[isVersionOf] Dirofilaria immitis infection and dirofilariosis : an animal model of human filariosis [URI]http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2010010511