Reaginic and hemagglutinating antibody production in dogs infected with Dirofilaria immitis

Reaginic and hemagglutinating antibody production in dogs infected with Dirofilaria immitis

Creators Hayasaki Mineo

The kinetics of reaginic and hemagglutinating antibody productions in dogs experimentally infected with Dirofilaria immitis was studied by passive cutaneous anaphylaxis reaction and indirect hemagglutination test. Reaginic antibody was first detected on the 65th day of infection, which coincided with the fourth molt of worm development. Although the antibody transiently disappeared on the 97th day, it reappeared, thereafter, and boosted when microfilaremia became evident. The reaginic activity of sera was completely deleted by heating at 56℃ for 60 min and by reduction and alkylation procedures. Sephadex G-200 gel filtration analysis indicates that reaginic activity was recovered in the ascending portion of the second (IgG) peak, suggesting molecular weight slightly greater than that of IgG. Hemagglutinating antibody was first observed on the l9th day of infection and then, steadily increased until the end of the study. Hemagglutinating antibody responses showed the pattern of two distinct peaks which coincided both with the fourth larval molt and the occurrence of microfilaremia.

[ISSN]0021-5295

[NCID]AN00191788

[isVersionOf] [URI]http://www.jstage.jst.go.jp/browse/jvms/-char/ja

[isVersionOf] Dirofilaria immitis infection and dirofilariosis : an animal model of human filariosis [URI]http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2010010511