Further Studies on the Distribution of Trypsin Inhibitors in the Proteins of Various Avian Egg Whites II. Cross Diagrams of Egg Whites of Various Birds

諸鳥類卵白中のトリプシン・インヒビターの分布に関する研究 第2編 諸鳥類卵白の交叉図形

Further Studies on the Distribution of Trypsin Inhibitors in the Proteins of Various Avian Egg Whites II. Cross Diagrams of Egg Whites of Various Birds

Creators Nagao Minoru

The egg white proteins of 16 species of bird were studied. The cross diagrams against trypsin and the distribution curves of trypsin inhibiting activities were compared. Following results were obtained. 1) The cross diagram formed one oｒ two peaks at the crossing points with ovomucoid and conalbumin. 2) A second trypsin inhibitor appeared in the egg whites of some birds (quail, long tail fowl, kite, owl and ostrich) in or near the region of conalbumin. 3) Trypsin inhibiting activity of each fraction isolated by sectioning the paper electropherogram was measured using benzoylarginine p-nitroanilide and chymotrypsin inhibiting activity was measured using benzoyltyrosine p-nitroanilide. 4) The cross diagram and the distribution curve of inhibitor of some birds (quail, turkey, fantail and penguin) formed a major peak in the region of ovalbumin. In these cases the ovomuciod seems to migrate with a mobility similar to that of ovalbumin. 5）The distribution　curve of chymotrypsin was measured only with the egg whites of the quail and the golden pheasant. In the case of the quail, the peak of chymotrypsin inhibitor in the distribution curve corresponded to the minor second peak of the cross diagram against trypsin. The curve of the golden pheasant egg white coincided with the cross diagram against trypsin as well as with that against chymotrypsin.

[ISSN]0513-1731

[NCID]AN00243156