Safety and influence of molecular hydrogen/carbon dioxide contained drinking water on common anthropometric and laboratory parameters

水素/炭酸ガス含有飲用水の各種生体計測値への影響と安全性の検討

Safety and influence of molecular hydrogen/carbon dioxide contained drinking water on common anthropometric and laboratory parameters

Creators Ikeda Yasuhiro

Creators Muroya Satomi

Creators Satou Kazutaka

Creators Inoue Goichi

Creators Koike Kunihiko

Creators Maekawa Tsuyoshi

近年,分子状水素には強い抗酸化作用のあることが多数報告され,蘇生後脳症や急性心筋梗塞の虚血後再灌流では過酸化物質が発生して組織障害を起こす.分子状水素はこの新しい治療法として注目されている.一方,健常人への水素ガス投与効果については,まだ詳しく検証されていない.本研究では健常者ボランティアを対象に1000mL/日の炭酸ガス含有飲用水(CO_2摂取期間),または水素/炭酸ガス含有飲用水(H_2/CO_2摂取期間)を各々31日間の飲用のうち26日間以上摂取できた者で比較し,P<0.05を目安に統計的有意性を判定した.生理学的諸量の摂取期間前後比較では,CO_2摂取期間で飲用後にSPO_2が平均0.43%有意に増加した.H_2/CO_2摂取期間では飲用後に収縮期血圧,拡張期血圧がそれぞれ5.2,2.8mmHg有意に減少し,終末呼気水素濃度は平均2.4ppm上昇したが有意ではなかった.生化学検査および血球検査の飲用前後比較では,CO_2摂取期間で飲用後に総ビリルビン,コリンエステラーゼ,γGTP,CK-MB,Ca,赤血球,血小板等が統計学的に有意に減少した.しかし,既知の個体内変動係数(CV_I)から許容変化幅(RCV)を求めて対比判定すると,何れの変化もRCV内であった.また,H_2/CO_2摂取期間では飲用後にγGTP,CK-MB等が有意に減少したが,同じくその変化量はRCV内であった.一方,CO_2摂取後の変化量とH_2/CO_2摂取後の変化量を比較すると,後者で脈拍数と総ビリルビンが有意差(p<0.05)に増加したがその変化量はRCV内であり,変化は生理的な変動範囲内であった.結論として,生理学的検査ではH_2/CO_2含有飲用水を一ヵ月摂取後に,収縮期血圧,拡張期血圧が統計学的に有意に低下し,CO_2含有飲用水ではSPO_2が軽度有意に増加した.一方,臨床検査値は各飲用水摂取後に有意な変化を示す項目は多かったが,何れもRCVとの対比では許容変化内の軽微なものであり,H_2/CO_2含有飲用水の人体への安全性に問題はないと判断された.今後,高濃度水素炭酸水や飲用期間を延長した条件で検討する必要がある.

Background:Recently, it has been shown that hydrogen gas has a strong antioxidant effect. Therefore, molecular hydrogen is drawing attention as a new therapeutic strategy against tissue damage caused by peroxides in post-ischemic reperfusion injury. Methods:In the present study, we investigated the effect of consuming 1000 ml/day of either carbonated water(CO_2-water)or hydrogen-molecule-added carbonated water(H_2/CO_2-water)for 26 days on physiological and laboratory parameters. Thirty-nine healthy volunteers were recruited and put on either of the water regimens using a cross-over design with 31-day washout period. Results:Among physiological parameters, SPO_2 significantly increased by 0.43% after drinking CO_2-water, whereas after drinking H_2/CO_2-water, systolic and diastolic blood pressure decreased significantly by 5.2 and 2.8 mmHg, respectively, and H_2 concentrations in end-expiratory air increased by 38% although statistically not significant. As for the biochemical and blood cell parameters, by the CO_2-water regimen, total bilirubin, γ-GTP, cholinesterase, CK-MB, Ca, erythrocyte and platelet counts significantly decreased(P<0.01).However, the differences were all found small when compared to the allowable limit of within-individual variation of each parameter, called reference change value(RCV).Whereas by the H_2/CO_2-water regimen, γ-GTP and CK-MB significantly decreased(P<0.01),but the differences were again within the RCV, and thus regarded as not clinically significant. Comparison of differences between the two regimens showed that only heart rate and total bilirubin were significantly higher by the H_2/CO_2 regimen, but of minor degree. Conclusion:Reduction of blood pressures observed in the H_2/CO_2-water regimen may represent an antioxidant effect of hydrogen molecule in water, although an additional trial by increasing H_2 concentration and/or longer drinking period is required to fortify the finding. As for the influences on other physiological and laboratory parameters, statistical differences were observed in several parameters by either of the regimens. However, in comparison to the RCV, the changes were within physioの意思決定支援に関する文献検討logical ranges, and thus there was no safety concern in drinking either of the water preparations.

[ISSN]0513-1731

[NCID]AN00243156