Variation with age in the numbers of ovulated ova and follicles of Wistar-Imamichi adult rats superovulated with eCG-hCG

Variation with age in the numbers of ovulated ova and follicles of Wistar-Imamichi adult rats superovulated with eCG-hCG

Creators Kagabu Satosi

Creators Umezu Motoaki

There are large variations with age in the number of ovulated ova found in superovulated female Wistar-Imamichi rats. In this study we investigated the numbers of ovulated ova and follicles with the aim of developing a superovulation technique that minimises variations. We also examined the number of non-atretic follicles in untreated rats aged 7-14 weeks, for each week of age. The numbers of 250-549 μm non-atretic follicles in untreated rats and the numbers of ovulated ova in superovulated rats both reached a peak at 12 weeks of age. The coefficients of variation for both follicle numbers and ova numbers changed with each week of age, reaching a maximum at 9 weeks of age and a minimum at 12 weeks of age. In order to achieve stable numbers of ova from superovulated rats, satisfactory results will be achieved using 12-week-old rats, minimising individual variations, with high numbers of ova.

[ISSN]1341-1357

[NCID]AA11032321

https://doi.org/10.1538/expanim.55.45

[isVersionOf] [URI]http://www.jstage.jst.go.jp/browse/expanim/-char/ja/