Studies on the Reversed Passive Latex Agglutination for the Detection of Enterotoxin : Part 2. Detection of Clostridium perfringens Enterotoxin
山口医学 Volume 33 Issue 4
Page 227-234
published_at 1984-08
Title
逆受身ラテックス凝集反応によるエンテロトキシン検出法の研究 : 第2報ウテルシュ菌エンテロトキシンについての研究
Studies on the Reversed Passive Latex Agglutination for the Detection of Enterotoxin : Part 2. Detection of Clostridium perfringens Enterotoxin
Creators
Sugiyama Junichi
Source Identifiers
Reversed passive latex agglutination (RPLA) for the detection of Clostridium perfringens enterotoxin was studied. For the sensitization of latex, immunoglobulins against the enterotoxin were purified by affinity chromatography. A suitable dose of immunoglobulin for sensitization of 0.5% latex suspension was the same amount of 25~50μg/ml. It gave detectable agglutination reactions at a concentration of 4μg/ml of the purified Clostridium perfringens enterotoxin as well as with cultures of some C. perfringens grown in Duncan-Strong (DS) medium. The detection of enterotoxin in patient's feces was also possible. The technical procedures of this method is very simple and easy. Also, the results are reliable. The sensitized latex is considered to be stable for at least 1 year when stored at 4℃. Therefore, this method can be recommended for the tests of C. perfringens enterotoxin in bacteriology, clinical bacteriology as well as epidemiology of acute enteritis.
Languages
jpn
Resource Type
journal article
Publishers
山口大学医学会
Date Issued
1984-08
File Version
Not Applicable (or Unknown)
Access Rights
metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools
医学部