The novel ribozyme, maxyzime, that inhibites leukemia progression in disease mouse
The bulletin of the Yamaguchi Medical School Volume 50 Issue 1-4
Page 1-5
published_at 2003-12
Title
The novel ribozyme, maxyzime, that inhibites leukemia progression in disease mouse
Creators
Tanabe Tsuyoshi
Source Identifiers
Creator Keywords
ribozyme
maxizyme
bcr-abl
leukemia
We have constructed an allosterically controllable novel enzyme (designared maxizyme) that can be transcribed in vivo under the control of a human tRNAVal promoter. The maxizyme has sensor arms that can recognize target sequences, and in the presence of such a target sequence only, it can from a cavity that cancapture catalytically indispensable Mg2+ ions. As a target for a demonstration of the potential utility of the maxizyme, we chose BCR-ABL mRNA, the translated products of which cause chronic myelogenous leukemia. Only the maxizyme (but not conventional ribozymes) had extremely high specificity and high-level activity, not only in vitro but also in cultured cells including BV173 cells derived from a patient with a Philadelphia chromosome. We have also found that this ribozyme complately inhibits tumor-cell infiltration in mice disease model.
Languages
eng
Resource Type
departmental bulletin paper
Publishers
Yamaguchi University Graduate School of Medicine
Date Issued
2003-12
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools
医学部