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The novel ribozyme, maxyzime, that inhibites leukemia progression in disease mouse

The bulletin of the Yamaguchi Medical School Volume 50 Issue 1-4 Page 1-5
published_at 2003-12
A020050000101.pdf
[fulltext] 223 KB
Title
The novel ribozyme, maxyzime, that inhibites leukemia progression in disease mouse
Creators Tanabe Tsuyoshi
Source Identifiers
Creator Keywords
ribozyme maxizyme bcr-abl leukemia
We have constructed an allosterically controllable novel enzyme (designared maxizyme) that can be transcribed in vivo under the control of a human tRNAVal promoter. The maxizyme has sensor arms that can recognize target sequences, and in the presence of such a target sequence only, it can from a cavity that cancapture catalytically indispensable Mg2+ ions. As a target for a demonstration of the potential utility of the maxizyme, we chose BCR-ABL mRNA, the translated products of which cause chronic myelogenous leukemia. Only the maxizyme (but not conventional ribozymes) had extremely high specificity and high-level activity, not only in vitro but also in cultured cells including BV173 cells derived from a patient with a Philadelphia chromosome. We have also found that this ribozyme complately inhibits tumor-cell infiltration in mice disease model.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 2003-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部