Combined Effects of a Metabotropic Glutamate Receptor Agonist and an Ionotropic Glutamate Receptor Agonist on Cytosolic Ca2+ Concentrations in Cultured Hippocampal Cells
The bulletin of the Yamaguchi Medical School Volume 42 Issue 1-2
Page 21-27
published_at 1995-12
Title
Combined Effects of a Metabotropic Glutamate Receptor Agonist and an Ionotropic Glutamate Receptor Agonist on Cytosolic Ca2+ Concentrations in Cultured Hippocampal Cells
Creators
Nagatsugu Yoshio
Source Identifiers
Creator Keywords
trans-ACPD
AMPA
NMDA
fura-2
cytosolic calcium concentration
Changes in the cytosolic Ca2+ concentration ([Ca2+]i) of cultured hippocampal cells from rat embryos were investigated using a fluorescent Ca2+ indicator, fura-2. An ionotropic and a metabotropic glutamate receptor agonist were applied singly or simultaneously to the same cell and Δ[Ca2+]i (the maximum increase in [Ca2+]i) after each application was calculated. In cells that responded well to 30 μM N-methy1-D-aspartate (NMDA), Δ[Ca2+]i induced by simultaneous application of trans-1-amino-cyclopenty1-1, 3-dicarboxylic acid (trans-ACPD) and α-amino-3-hydroxy-5-methy1-4-isoxazoleprppionic acid (AMPA) was 101.2±40.3 nM, whereas the sum of Δ[Ca2+]i values induced by these agents alone was 87.6±32.2 nM (p=0.061, n=55). In 18% of these cells, yhe mean Δ[Ca2+]i evoked by the simultaneous application of AMPA and trans-ACPD was at least twice the mean sun of Δ[Ca2+]i values evoked by these agents alone. In cells which only responded to 30 μM NMDA with small Δ[Ca2+]I changes, Δ[Ca2+]I induced by simultaneous application of AMPA and trans-ACPD was significantly higher than the sum of Δ[Ca2+]I values induced by these agants alone, 130.1±35.2 and 41.2±24.0 nM, respectively (p<0.001, n=17). These results suggest that activation of metabotropic glutamate receptors upregulates AMPA receptors in hippocampal cells.
Languages
eng
Resource Type
departmental bulletin paper
Publishers
Yamaguchi University Graduate School of Medicine
Date Issued
1995-12
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools
医学部