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Lentivirus Vector Mediated Hematopoietic Stem Cell Gene Transfer of Common Gamma-Chain Cytokine Receptor in Rhesus Macaques

The bulletin of the Yamaguchi Medical School Volume 51 Issue 3-4 Page 37-40
published_at 2004-12
A020051000302.pdf
[fulltext] 113 KB
Title
Lentivirus Vector Mediated Hematopoietic Stem Cell Gene Transfer of Common Gamma-Chain Cytokine Receptor in Rhesus Macaques
Creators Dong Sung An.
Source Identifiers
We examined the efficiency of marking, gene expression, and transplant of bone marrow and peripheral blood CD34 +cells using a lentivirus vector in non-human primate model systems. In vitrocytokine stimulation was not required to achieve efficient transduction of CD34 +cells resulting in marking and gene expression of the reporter gene encoding enhanced green fluorescent protein (EGFP) following transplant of the CD34 +cells. Monkeys transplanted with mobilized peripheral blood CD34 +cells resulted in EGFP expression in 1 to 10% of multilineage peripheral blood cells, including red blood cells and platelets, stable for 5 years to date. The relative level of gene expression utilizing a lentiviral vector bearing a RhMLV promoter is 2- to 10-fold greater than that utilizing a lenntivirus vector bearing the cytomegalovirus immediate-early promoter. In contrast, in animals transplanted with autologous bone mallow CD34 +cells, multilineage EGFP expression was evident initially but diminished over time. We further tested our lentivirus vector system by demonstrating gene transfer of the human common gamma-chain cytokine receptor gene (νc), deficient in X-linked SCID patients and recently successfully used to treat disease. Marking was 0.42 and 0.001 HIV-1 vector DNA copy per 100 cells in two animals. To date, all EGFP- and νc-transplanted animals are healthy. This system may prove useful for expression of therapeutic genes in human hematopoientic cells.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 2004-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部