A Rapid and Simple Ultramicromethod for the Estimation of Serum Pseudocholinesterase with Butyrylthiocholine as Substrate
The bulletin of the Yamaguchi Medical School Volume 15 Issue 4
Page 257-268
published_at 1968-12
Title
A Rapid and Simple Ultramicromethod for the Estimation of Serum Pseudocholinesterase with Butyrylthiocholine as Substrate
Creators
Nakashima Koji
Creators
Sera Yoko
Creators
Nakano Kazuko
Source Identifiers
A simple and rapid ultramicromethod for the determination of serum pseudocholinesterase with butyrylthiocholine as substrate was devised. Its principle consists in reading the initial velocity of the reaction of DTNB with thiocholine which is enzymatically released from butyrylthiocholine at 37 ℃. The production rate of the chromogenic substance (5-mercapto-2-nitrobenzoic acid), namely the time (in seconds) necessary for causing an increment of 0.10 in absorbance is measured at 410 mμ, in a photoelectric colorimeter equipped with a cuvette holder and a drain system warmed at constant temperature (37℃). The method is suitable not only routine clinical chemistry but also for emergency clinic. It requires minimal equipment, specimens, pipetting and time, but has satisfactory precision and accuracy.
Languages
eng
Resource Type
departmental bulletin paper
Publishers
Yamaguchi University Graduate School of Medicine
Date Issued
1968-12
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools
医学部