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Heat Denaturation Test for Unstable Hemoglobin, with a Note on Its Application to Hemoglobin Survey in Japan

The bulletin of the Yamaguchi Medical School Volume 16 Issue 2-4 Page 183-194
published_at 1969-12
A020016000209.pdf
[fulltext] 1.1 MB
Title
Heat Denaturation Test for Unstable Hemoglobin, with a Note on Its Application to Hemoglobin Survey in Japan
Creators Ueda Satoshi
Creators Matsuoka Miyoko
Creators Miyaji Takaoki
Creators Ueda Naoki
Creators Aoki Kenzo
Creators Izawa Michi
Creators Shibata Susumu
Source Identifiers
 Dacie's heat denaturation test was modified in order to get a practical procedure for detecting unstable hemoglobin in hemolysates. For the purpose of screening a centrifugation method was devised : hemolysate, phosphate buffer solution (0.1 M, pH 7.4), and zinc chloride solution were mixed in equal proportions (0.2 ml each) in a test tube and transferred to a capillary tube (0.3 × 12 cm). The capillary tube was sealed at one end by fusion in a flame of a burner so that the mixture might be contained safely in it, warmed at 50℃ for 60 minutes, and then centrifuged at 3000 rpm (1700 G) for 5 minutes to read the amount of precipitate sedimented at the bottom in terms of the percentage of the total co1umn of the mixture. A reading larger than 3.5 per cent is suggesive of the presence of unstable hemoglobin in hemolysate. Such a hemolysate should be examined by spectrophotometric method to connrm the result. Namely, hemolysate (30 μl) was mixed with warm (55℃) phosphate buffer solution (0.1 M, pH 7.4
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1969-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部