Studies on the Technical Procedures in Scanning Electron Microscopy : 2. Scanning Electron Microscopic Method for Spirality of Short-type Bacteria, with Special Reference to Preparation

In order to investigate a twisting body of the short-type bacteria, the elongation method using Cephalexin was attempted in scanning electron microscopy. Two species of Spirillum and two species of vibrio were used, and Spirillum itersonii with definite spiral was also used for the positive control and Escherichia coli without spiral for the negative control, respectively. Two bacteria for controls preserved their shapes with or without spirality in the media containing Cephalexin. Other spirilla and vibrio provided the elongated cell bodies around concentration of MIC and demonstrated definite spiral-direction. Therefore, this method was considered to be useful for investigation of the twisted body of the short-type becteria.