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Allogenic multilayered fibroblast sheets promote anastomotic site healing in a rat model of esophageal reconstruction

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Title
他家積層線維芽細胞シートによるラットの食道縫合部の補強効果
Allogenic multilayered fibroblast sheets promote anastomotic site healing in a rat model of esophageal reconstruction
Degree 博士(医学) Dissertation Number 医博甲第1684号 (2023-09-26)
Degree Grantors Yamaguchi University
[kakenhi]15501 grid.268397.1
Abstract
Objective: Anastomotic leakage is a common and severe complication of esophageal reconstruction. Accordingly, there is a clinical need for novel methods to prevent it. We developed multilayered, growth factor-secreting fibroblast sheets that promote wound healing and angiogenesis. The present study aimed to assess the utility of allogenic multilayered fibroblast sheets in preventing esophageal anastomotic leakage in a rat model of esophageal reconstruction. Methods: Allogenic multilayered fibroblast sheets prepared from oral mucosal tissues were implanted at esophageal anastomotic sites. Results: The allogenic multilayered fibroblast sheet group had significantly higher burst pressure and collagen deposition compared to a control group five days postoperatively. The expression levels of collagen type I and III mRNAs around esophageal suture sites were higher in the allogenic multilayered fibroblast sheet group compared to the control group on postoperative days 0, 3, and 5. There was a trend toward lower anastomotic leakage and lower abscess scores in the allogenic multilayered fibroblast sheet group compared to the control group; however, these differences did not reach statistical significance. Allogenic multilayered fibroblast sheets completely disappeared at ten days after implantation. Further, no inflammation was observed at suture sites with implanted allogenic multilayered fibroblast sheets at five days after surgery. Conclusion: Allogenic multilayered fibroblast sheets may represent a promising method of preventing esophageal anastomotic leakage.
Creators 山本 直宗
Creator Keywords
Regenerative medicine allogenic multilayered fibroblast sheet sheet implantation anastomotic site healing proliferation of granulation tissue
Languages eng
Resource Type doctoral thesis
File Version Version of Record
Access Rights open access
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[references] [PMID]37303629