- 部局一覧
- 大学院医学系研究科(医学)
大学院医学系研究科(医学)
Date Issued (<span class="translation_missing" title="translation missing: en.view.desc">Desc</span>)
Medical Science & Innovation Volume 70 Issue 1-2
pp. 1 - 6
published_at 2023-06
Catecholaminergic polymorphic ventricular tachycardia (CPVT) is caused by a single point mutation in the cardiac type 2 ryanodine receptor (RyR2). Using knock-in mouse (KI) model (R2474S/+), we previously reported that a single point mutation within the RyR2 sensitized the channel to agonists, primarily mediated by defective inter-domain interaction within the RyR2 and subsequent dissociation of calmodulin (CaM) from the RyR2. Here, we examined whether CPVT can be genetically rescued by enhancing the binding affinity of CaM to the RyR2. We first determined whether there was a possible amino-acid substitution within the CaM-binding domain in the RyR2 (3584-3603) that can enhance its binding affinity to CaM, and found that V3599K substitution showed the highest binding affinity of CaM to CaM-binding domain. Hence, we generated a heterozygous KI mouse model (V3599K/+) with a single amino acid substitution in the CaM-binding domain of the RyR2, and crossbred it with the heterozygous CPVT –associated R2474S/+ KI mouse to obtain a double heterozygous R2474S/V3599K KI mouse model. The CPVT phenotypes, bidirectional or polymorphic ventricular tachycardia, were inhibited in the R2474S/V3599K mice. Thus, enhancement of the CaM binding affinity of the RyR2 is essential to prevent CPVT-associated arrhythmogenesis.
Creators :
Nakamura Yoshihide
Yamamoto Takeshi
Kobayashi Shigeki
Yano Masafumi
Publishers : Yamaguchi University School of Medicine
Medical Science & Innovation Volume 70 Issue 1-2
pp. 7 - 17
published_at 2023-06
To investigate whether dantrolene (DAN), cardiac ryanodine receptor (RyR2) stabilizer, improves impaired diastolic function in an early pressure-overloaded hypertrophied heart, pressure-overload hypertrophy was induced by transverse aortic constriction (TAC) in mice. Wild-type (WT) mice were divided into four groups: sham-operated mice (Sham), sham-operated mice treated with DAN (DAN+Sham), TAC mice (TAC), and TAC mice treated with DAN (DAN+TAC). The mice were then followed up for 2 weeks. Left ventricular (LV) hypertrophy was induced in TAC, but not DAN+TAC mice, 2 weeks after TAC. There were no differences in LV fractional shortening among the four groups. Catheter tip micromanometer showed that the time constant of LV pressure decay, an index of diastolic function, was significantly prolonged in TAC but not in DAN+TAC mice. Diastolic function was significantly impaired in TAC, but not in DAN+TAC mice as determined by cell shortening and Ca^{2+} transients. An increase in diastolic Ca^{2+} leakage and a decrease in calmodulin (CaM) binding affinity to RyR2 were observed in TAC mice, while diastolic Ca^{2+} leakage improved in DAN+TAC mice. Thus, DAN prevented the progression of hypertrophy and improved the impairment of LV relaxation by inhibiting diastolic Ca^{2+} leakage through RyR2 and the dissociation of CaM from RyR2.
Creators :
Chang Yaowei
Kobayashi Shigeki
Yano Yasutake
Uchida Tomoyuki
Nawata Junya
Fujii Shohei
Nakamura Yoshihide
Suetomi Takeshi
Uchinoumi Hitoshi
Oda Tetsuro
Yamamoto Takeshi
Yano Masafumi
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 69 Issue 3-4
pp. 45 - 56
published_at 2022-12
Abstract Purpose: The purpose of this study was to investigate the effects of two different postures (sitting and standing) and three different ambient temperatures (10℃, 20℃, and 30℃) on heart rate variability (HRV) among healthy young adults. Methods: Twelve young adult volunteers (males 6, females 6) were recruited. Following acclimatization to any the room temperature (10℃, 20℃ or 30℃), 5-min measurements of HRV were conducted in sitting and standing postures of the subjects. Results: Compared to the sitting posture, measurements obtained in the standing posture revealed a significant decrease in high-frequency power/HF, root mean square of successive differences between RR intervals, standard deviation of Poincaré plot perpendicular to the line-of-identity or SD1 and SD1/standard deviation of Poincaré plot along the line-of-identity or SD2, and a significant increase in lowfrequency power/LF and LF/HF under all experimental conditions (p<0.05 to 0.005). Majority of HRV parameters showed significant differences while the values obtained under 10℃ were compared with 20℃ and 30℃ conditions, respectively (p<0.05 to 0.001). Conclusions: Our findings suggest the predominance of sympathetic tone in the standing compared with sitting posture. Furthermore, colder conditions caused a predominance of the parasympathetic activity in both sitting and standing postures, and such effects of ambient temperature on the sympathovagal balance were stronger in the latter posture.
Creators :
Wada Sunao
Mahbub MH
Nakagami Yuki
Hase Ryosuke
Yamaguchi Natsu
Takahashi Hidekazu
Saito Hiroyuki
Shimokawa Junki
Watanabe Rie
Harada Noriaki
Tanabe Tsuyoshi
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 69 Issue 3-4
pp. 27 - 35
published_at 2022-12
Abstract Objectives: This study examined the short-term outcomes of endovascular therapy (EVT) with the GORE VIABAHN VBX (VBX) which is a balloon-expandable covered stent in patients with aortoiliac occlusive disease (AIOD) and acute limb ischemia (ALI). Methods: This was a single-center retrospective review of patients with AIOD and ALI who underwent EVT with the VBX between December 2018 and January 2021. Primary, assisted, and secondary patency rates were assessed, along with 30-day mortality rates. Results: Twenty-two patients underwent EVT with the VBX; 18 patients had AIOD, whereas 4 had ALI. The incidence of TransAtlantic Intersociety Classification II D lesions was 50% (n=9). Technical success was 100%. One patient with unilateral common iliac artery stenosis developed an intraoperative dissection of the distal edge of the VBX, which required an additional self-expanding VIABAHN stent on the distal edge of the VBX. The primary patency rate at 24 months was 100%. No patients required additional target vessel treatment at a median follow-up of 10 months (range, 1-27). EVT was also successful in the four patients with ALI, who had no complications of the target vessel. Conclusion: The present study demonstrated that EVT with the VBX is effective for AIOD and ALI.
Creators :
Mizoguchi Takahiro
Morikage Noriyasu
Ike Soichi
Nagase Takashi
Samura Makoto
Harada Takasuke
Kurazumi Hiroshi
Suzuki Ryo
Suehiro Kotaro
Hamano Kimikazu
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 69 Issue 1-2
pp. 11 - 19
published_at 2022-06
The hippocampal dentate gyrus has been identified to play a critical role in maintaining contextual memory in many mammalian species. To evaluate learninginduced synaptic plasticity of granule cells, we subjected male rats to an inhibitory avoidance (IA) task and prepared acute hippocampal slices. In the presence of 0.5 μM tetrodotoxin, we recorded miniature excitatory post synaptic currents (mEPSCs) and inhibitory post synaptic currents (mIPSCs) in male rats experiencing four groups: untrained, IA-trained, unpaired, and walk-through. Compared with the untrained, IA-trained, unpaired, and walk-through groups, the unpaired group significantly enhanced mean mEPSC amplitudes, suggesting the experience-induced plasticity at AMPA receptor-mediated excitatory synapses. For inhibitory synapses, both unpaired and walk-through groups significantly decreased mean mIPSC amplitudes, showing the experience-induced reduction of postsynaptic GABAA receptor-mediated currents. Unlike the plasticity at CA1 synapses, it was difficult to explain the learning- specific plasticity at the synapses. However, overall multivariate analysis using four variables of mE(I)PSC responses revealed experience-specific changes in the diversity, suggesting that the diversity of excitatory/inhibitory synapses onto granule cells differs among the past experience of animals include the learning. In comparison with CA1 pyramidal neurons, granule cells consistently showed greater amplitude and frequency of mE(I)PSCs. Fluctuation analysis further revealed that granule cells provide more postsynaptic AMPA receptor channels and greater single-channel current of GABAA receptors of than CA1 pyramidal neurons. These findings show functional differences between two types of principal cells in the hippocampus.
Creators :
Han Thiri Zin
Sakimoto Yuya
Mitsushima Dai
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 69 Issue 1-2
pp. 21 - 25
published_at 2022-06
Incontinentia pigmenti (IP; OMIM# 308300) is a rare inherited disease caused by a mutation of IKBKG, which is also known as NEMO, located on Xq28. IP patients usually present with abnormalities of skin, eyes, nails and central nervous system. The neurological damege, which occur usually from neonatal through the early infantile period, leads to neulogical complication such as development retardation, motor paralysis and epilepsy. However, there has been little study done concerning the effect of therapy for the neural abnormalities. We have investigated the clinical findings in a female IP case with a novel mutation of IKBKG for six years due to disclosing the neurological prognosis and the immunological features. It is hoped that the present study will contribute to a better management of IP patients.
Creators :
Sakata Yasufumi
Hirano Reiji
Yasudo Hiroki
Shimomura Yutaka
Takada Hidetoshi
Suehiro Yutaka
Ohga Shouichi
Hasegawa Shunji
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 69 Issue 1-2
pp. 1 - 9
published_at 2022-06
Androgen receptor (AR)-signaling plays an important role in promoting tumor cell survival and the development of castration-resistant prostate cancer (CRPC). Androgen deprivation therapy is the basic therapeutic strategy for AR signaling blockade, but that effects wonʼt last long and most of metastatic prostate cancer show castration resistance. Recently, new agents, AR Axis Targeted agents (ARAT), have been developed. We have been developing our bench-to-bedside translational research to elucidate the mechanisms of drug resistance in prostate cancer. This review describes our investigational results and recent advances in CRPC with ARAT treatment strategy.
Creators :
Matsumoto Hiroaki
Matsuyama Hideyasu
Publishers : Yamaguchi University School of Medicine
山口医学 Volume 70 Issue 4
pp. 149 - 156
published_at 2021-12-02
山口医学 Volume 70 Issue 4
pp. 157 - 164
published_at 2021-12-02
山口医学 Volume 70 Issue 3
pp. 89 - 98
published_at 2021-09-01
We performed this study to investigate whether serum cell-free DNA testing(liquid biopsy)of either methylated SEPT9 or methylated SST, which are possible biomarkers of HCC, in combination with AFP may improve clinical performance for the detection of HCC. Study subjects included 25 healthy controls, 15 patients with chronic liver disease without HCC, and 38 patients with HCC. Methylation level of serum cell-free SEPT9 and SST was measured by methylation-sensitive restriction enzymes followed by multiplex droplet digital polymerase chain reaction. According to receiver operating characteristic curve analysis to discriminate between non-HCC group(including control group and chronic liver disease without HCC group)and HCC group, area under the curve(AUC)was 0.73 by AFP and 0.73 by mSEPT9, whereas it was only 0.59 by mSST. Regarding combination tests, although negative predictive values were almost same between the AFP + mSEPT9 test and the AFP + mSST test(75.0% vs 72.2%),a positive predictive value of AFP + mSEPT9 test was higher than that of the AFP + mSST test(79.4% vs. 66.7%).In conclusions, methylated SEPT9 was a better companion marker of AFP for HCC screening than methylated SST.
Creators :
Yamasaki Ayano
Suehiro Yutaka
Hoshida Tomomi
Saeki Issei
Yamauchi Kotoh Yurika
Matsumoto Toshihiko
Takami Taro
Sakaida Isao
Yamasaki Takahiro
Publishers : 山口大学医学会
山口医学 Volume 70 Issue 2
pp. 71 - 81
published_at 2021-05-13
Creators :
Fujimura Kazumi
Hase Ryosuke
Kijima Ayano
Okochi Ayako
Kumagai Tamaki
Publishers : 山口大学医学会
山口医学 Volume 70 Issue 2
pp. 65 - 69
published_at 2021-05-13
Creators :
Suetomi Takeshi
Publishers : 山口大学医学会
山口医学 Volume 70 Issue 1
pp. 45 - 52
published_at 2021-03-04
Creators :
Zhang Min
Publishers : 山口大学医学会
山口医学 Volume 70 Issue 1
pp. 29 - 35
published_at 2021-03-04
Creators :
Miyanari Kenji
Publishers : 山口大学医学会
山口医学 Volume 70 Issue 1
pp. 53 - 58
published_at 2021-03-04
山口医学 Volume 70 Issue 1
pp. 37 - 43
published_at 2021-03-04
The bulletin of the Yamaguchi Medical School Volume 68 Issue 1-2
pp. 11 - 20
published_at 2021
Aim: To evaluate the efficacy of quantitative analysis for differentiating pancreatic ductal adenocarcinoma (PDAC) and inflammatory pancreatic masses (IPM) using time intensity curve (TIC) analysis based on contrast-enhanced endoscopic ultrasonography (CE-EUS). Methods: We reviewed 89 patients who had undergone CE-EUS for pancreatic solid lesions at our department between August 2012 and January 2016. CE-EUS images were recorded for 2 minutes after injection of the contrast agent. The diagnostic abilities of the enhanced patterns and TIC analysis were assessed. Results: The enhanced patterns of PDAC were mainly hypovascular and heterogeneous (66/77), while IPM were mainly isovascular and homogeneous (6/12). In PDAC, sensitivity was 77.9%, specificity 83.3%, and accuracy was 78.7%. In TIC analysis, the intensity reduction rate was significantly different at 10 and 30 seconds after peak intensity. After creating a cutoff value (49%) based on the receiver operating characteristic curve for the intensity reduction rate after 30 seconds, diagnosing PDAC with TIC analysis had a sensitivity of 67.5%, specificity of 100%, and accuracy of 71.9%. Combining enhanced pattern analysis with TIC analysis had a sensitivity of 90.9%, pecificity of 83.3%, and accuracy of 89.9%. Conclusion: Combining TIC analysis with CE-EUS improved diagnostic accuracy when differentiating between PDAC and IPM.
Creators :
Yasuda Mayumi
Kaino Seiji
Tsuyama Takanori
Fujimoto Yuko
Amano Shogo
Harima Hirofumi
Suenaga Shigeyuki
Sakaida Isao
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 68 Issue 1-2
pp. 21 - 29
published_at 2021
Creators :
Mahbub MH
Yamaguchi Natsu
Hase Ryosuke
Sunagawa Hiroshi
Ishimaru Yasutaka
Yamamoto Hiroshi
Kikuchi Shinya
Tanabe Tsuyoshi
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 68 Issue 1-2
pp. 5 - 10
published_at 2021
The bulletin of the Yamaguchi Medical School Volume 68 Issue 3-4
pp. 31 - 39
published_at 2021
Creators :
Ishimaru Yasutaka
Mahbub MH
Yamaguchi Natsu
Hase Ryosuke
Nakagami Yuki
Takahashi Hidekazu
Watanabe Rie
Saito Hiroyuki
Shimokawa Junki
Yamamoto Hiroshi
Kikuchi Shinya
Tanabe Tsuyoshi
Publishers : Yamaguchi University School of Medicine
山口医学 Volume 69 Issue 4
pp. 161 - 168
published_at 2020-12-25
山口医学 Volume 69 Issue 3
pp. 141 - 144
published_at 2020-09-25
Creators :
Yano Yuka
Kuga Takayuki
Sakamoto Ryuunosuke
Shigeta Masatoshi
Iida Yasuhiko
Publishers : 山口大学医学会
山口医学 Volume 69 Issue 2
pp. 75 - 80
published_at 2020-05-01
山口医学 Volume 69 Issue 1
pp. 5 - 11
published_at 2020-02-28
The bulletin of the Yamaguchi Medical School Volume 67 Issue 1-2
pp. 1 - 10
published_at 2020
Creators :
Aibe Yuki
Takami Taro
Fujisawa Koichi
Matsumoto Toshihiko
Sakaida Isao
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 67 Issue 3-4
pp. 11 - 17
published_at 2020
The bulletin of the Yamaguchi Medical School Volume 67 Issue 3-4
pp. 19 - 23
published_at 2020
Creators :
Matsukuma Satoshi
Yoshimura Kiyoshi
Tsunedomi Ryouichi
Hazama Shoichi
Nagano Hiroaki
Publishers : Yamaguchi University School of Medicine
山口医学 Volume 68 Issue 4
pp. 119 - 126
published_at 2019-12-11
Creators :
Murakami Junichi
Publishers : 山口大学医学会
山口医学 Volume 68 Issue 4
pp. 145 - 149
published_at 2019-12-11
Creators :
Nishimoto Arata
Hisanaga Takuro
Katsura Shunsaku
Fujimiya Tatsuya
Shirasawa Bungo
Publishers : 山口大学医学会
Acta criminologiae et medicinae legalis Japonica Volume 85 Issue 4
pp. 111 - 122
published_at 2019-12
山口医学 Volume 68 Issue 2-3
pp. 75 - 80
published_at 2019-08-09
Creators :
Hisanaga Takuro
Katsura Shunsaku
Nishimoto Arata
Fujimiya Tatsuya
Shirasawa Bungo
Publishers : 山口大学医学会
山口医学 Volume 68 Issue 1
pp. 31 - 37
published_at 2019-03-01
Creators :
Sunagawa Hiroshi
Publishers : 山口大学医学会
山口医学 Volume 68 Issue 1
pp. 23 - 29
published_at 2019-03-01
山口医学 Volume 68 Issue 1
pp. 39 - 46
published_at 2019-03-01
Creators :
Nakamura Takashi
Sakano Hisashi
Kudo Junichi
Matsumoto Masaharu
Nakamura Munetaka
Yoneshiro Shigeru
Kuga Takayuki
Itoh Hiroshi
Hamano Kimikazu
Publishers : 山口大学医学会
山口医学 Volume 68 Issue 1
pp. 13 - 22
published_at 2019-03-01
Journal of higher education Volume 16
pp. 76 - 79
published_at 2019-03
Creators :
Yamamoto Naoki
Morifuku Orie
Umemoto Tomoko
Fujikatsu Ayaka
Harada Yukiko
Nakahara Atsuko
Hatano Hiromi
Kobayashi Kumi
Sumida Tomoko
Morimoto Hiroshi
Okuya Shigeru
Matsubara Toshio
Publishers : 山口大学大学教育機構
The bulletin of the Yamaguchi Medical School Volume 66 Issue 3-4
pp. 45 - 51
published_at 2019
Creators :
Ozaki Masakazu
Kouda Kyoji
Furukawa Hiroyuki
Kitahara Takashi
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 66 Issue 1-2
pp. 1 - 6
published_at 2019
Creators :
Kato Takayoshi
Yamamoto Takeshi
Kobayashi Shigeki
Yano Masafumi
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 66 Issue 1-2
pp. 7 - 13
published_at 2019
Creators :
Fukui Yumi
Saeki Issei
Matsuda Takashi
Maeda Masaki
Hisanaga Takuro
Iwamoto Takuya
Matsumoto Toshihiko
Hidaka Isao
Ishikawa Tsuyoshi
Takami Taro
Yamasaki Takahiro
Sakaida Isao
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 66 Issue 1-2
pp. 29 - 35
published_at 2019
Creators :
Hanazono Tadasuke
Hidaka Isao
Maeda Masaki
Saeki Issei
Iwamoto Takuya
Ishikawa Tsuyoshi
Takami Taro
Yamasaki Takahiro
Sakaida Isao
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 66 Issue 1-2
pp. 15 - 27
published_at 2019
Creators :
Hisano Takahiro
Cui Dan
Harada Koji
Kimura Tokuhiro
Mishima Katsuaki
Ikeda Eiji
Publishers : Yamaguchi University School of Medicine
山口医学 Volume 67 Issue 4
pp. 187 - 192
published_at 2018-12-28
山口医学 Volume 67 Issue 1
pp. 43 - 48
published_at 2018-02-01
Creators :
Satou Youko
Fukamitu Gaku
Yamamoto Shigeru
Nagano Hiroaki
Hamano Kimikazu
Publishers : 山口大学医学会
山口医学 Volume 67 Issue 1
pp. 27 - 32
published_at 2018-02-01
Creators :
Suehiro Yuki
Harada Eijiro
Kawamura Daichi
Kugimiya Naruji
Takemoto Yoshihiro
Hamano Kimikazu
Publishers : 山口大学医学会
The bulletin of the Yamaguchi Medical School Volume 65 Issue 3-4
pp. 27 - 35
published_at 2018
Creators :
Nishimura Takashi
Okamura Takayuki
Tateishi Hiroki
Fujimura Tatsuhiro
Oda Tetsuro
Wada Yasuaki
Okuda Shinichi
Omuro Ayumi
Maeda Takako
Hamada Yoriomi
Nanno Takuma
Fukui Go
Yamada Jutaro
Yano Masafumi
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 65 Issue 1-2
pp. 21 - 25
published_at 2018
Creators :
Iida Yasunori
Hasegawa Shunji
Korenaga Yuno
Okada Seigo
Suzuki Yasuo
Hirano Reiji
Ohga Shouichi
Publishers : Yamaguchi University School of Medicine
Forensic pharmacokinetics of alcohol and its metabolites for the purpose of expert witness testimony
法医学の実際と研究 Volume 61
pp. 1 - 24
published_at 2018
The bulletin of the Yamaguchi Medical School Volume 65 Issue 1-2
pp. 11 - 20
published_at 2018
Creators :
Mahbub MH
Hase Ryosuke
Yamaguchi Natsu
Takahashi Hidekazu
Kawano Yoshinao
Hiroshige Keiichi
Tanabe Tsuyoshi
Harada Noriaki
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 65 Issue 1-2
pp. 1 - 10
published_at 2018
Creators :
Yoshida Hirokazu
Tanaka Nobuaki
Toyota Ayako
Kunimitsu Kenta
Wada Yasuaki
Fujii Ayano
Ariyoshi Toru
Okuda Shinichi
Yano Masafumi
Publishers : Yamaguchi University School of Medicine
山口医学 Volume 66 Issue 3
pp. 163 - 168
published_at 2017-08-01
Creators :
Hisanaga Takuro
Yamasaki Takahiro
Maeda Masaki
Iwamoto Takuya
Saeki Issei
Matsumoto Toshihiko
Hidaka Isao
Marumoto Yoshio
Ishikawa Tsuyoshi
Takami Taro
Sakaida Isao
Publishers : 山口大学医学会
山口医学 Volume 66 Issue 2
pp. 135 - 138
published_at 2017-05-01
山口医学 Volume 66 Issue 2
pp. 89 - 96
published_at 2017-05-01
Background The lipid-lowering therapy by statins may stabilize and reduce coronary plaques. We compare the effect of strong or moderate statins on the coronary plaque characteristics by using 64-slice multidetector computed tomography (MDCT). Methods and Results We analyzed 13 subjects with non-calcified coronary plaques(NCP)as determined by MDCT. Pitavastatin(PTV:2mg/day)or pravastatin(PRA:10mg/day)were randomly administered. MDCT were performed at 0, 6 and 12 months after lipid-lowering therapy. In PTV group(n=6),CT density of NCP increased by 35.6 ± 28.8 HU after 6 months(p=0.037)and by 30.6 ± 29.8 HU after 12 months(NS).NCP area was decreased by 35.9 ± 15.2 % after 6 months and by 41.0 ± 16.5 % after 12 months(both P=0.001).In PRA group(n=7),CT density of NCP did not significantly increased after 6 months(NS)and after 12 months(NS).NCP area was not significantly decreased at 6 months(NS),but decreased by 23.1 ± 16.7 % at 12 months(P=0.001). Conclusions Serial CT angiography revealed that the regression of NCP occurs rapidly by strong lipid lowering therapy compared to the moderate statin therapy.
Creators :
Nao Tomoko
Miura Toshiro
Yoshimura Masayuki
Fujimura Tatsuhiro
Nakashima Yoshiteru
Okada Munemasa
Matsunaga Naofumi
Yano Masafumi
Publishers : 山口大学医学会
山口医学 Volume 66 Issue 1
pp. 11 - 16
published_at 2017-02-01
山口医学 Volume 66 Issue 1
pp. 41 - 45
published_at 2017-02-01
Creators :
Sasaki Sho
Nishikawa Jun
Nagao Misato
Ogawa Ryo
Goto Atsushi
Hashimoto Shinichi
Okamoto Takeshi
Suzuki Chieko
Hoshii Yoshinobu
Sakaida Isao
Publishers : 山口大学医学会
A time and motion study of public health nurses’daily routine : Work breakdown and skill development
山口医学 Volume 66 Issue 1
pp. 17 - 24
published_at 2017-02-01
山口医学 Volume 66 Issue 1
pp. 5 - 10
published_at 2017-02-01
Creators :
Nagata Chizuru
Publishers : 山口大学医学会
The bulletin of the Yamaguchi Medical School Volume 64 Issue 1-2
pp. 1 - 12
published_at 2017
Aberrant increases in protein phosphatase 1(PP1) activity have been shown to be associated with inefficient sarcoplasmic reticulum Ca^{2+} cycling, leading to cardiac dysfunction in the failing heart. In the present study, we investigated whether BNP promoter-inducible suppression of PP1β would ameliorate progression of pressure overload-induced heart failure in mice, a clinically relevant animal model. An Adeno-associated virus 9 (AAV9) vector encoding PP1βshRNA and a negative control (NC) shRNA driven by a brain natriuretic peptide (BNP) promoter with an emerald green fluorescent protein expression (EmGFP) cassette were used to test the hypothesis. AAV9 vectors (AAV9-BNP-EmGFP-PP1βshRNA and AAV9-BNP-EmGFP-NCshRNA) were introduced into the in vivo heart via the tail vein injection (4x10^{11} GC/mice) in 8-week-old C57BL6J mice, followed by transverse aortic constriction (TAC) 2 weeks after the AAV9 vector injection. Post TAC cardiac function was sequentially assessed every 2 week by echocardiography, followed by hemodynamic assessment at 1 month. AAV9-BNP-EmGFP-PP1βshRNA treatment suppressed myocardial PP1β expression by 15% compared with the NCshRNA group (p<0.001). The fractional shortening (%FS) of the left ventricle in the PP1βshRNA-treated group was significantly larger than the NCshRNA-treated group (21%±1.0% vs. 15%±0.01, p<0.01). The ratios of heart weight (HW) / body weight (BW) and lung weight (LW) / BW in the PP1βshRNA group were significantly smaller than those of the NCshRNA group (HW/BW: 9.20±0.49 vs. 10.6±0.45 mg/g
Creators :
Shiraishi Kozo
Ikeda Yasuhiro
Miyazaki Yosuke
Fujimoto Shizuka N.
Yoshimura Koichi
Miura Toshiro
Matsuzaki Masanori
Yano Masafumi
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 64 Issue 3-4
pp. 13 - 19
published_at 2017
The bulletin of the Yamaguchi Medical School Volume 64 Issue 3-4
pp. 21 - 26
published_at 2017
Creators :
Kanchiku Tsukasa
Suzuki Hidenori
Imajo Yasuaki
Moriya Atsushi
Suetomi Yutaka
Nishida Norihiro
Funaba Masahiro
Takahashi Youhei
Taguchi Toshihiko
Publishers : Yamaguchi University School of Medicine
山口医学 Volume 65 Issue 4
pp. 161 - 166
published_at 2016-11-01
Creators :
Murata Yoriyuki
Aoe Keisuke
Utsunomiya Toshiaki
Sakamoto Kenji
Oishi Keiji
Kohtoku Wataru
Chikamori Kenichi
Maeda Tadashi
Okabe Kazunori
Murakami Tomoyuki
Yano Masafumi
Ueoka Hiroshi
Publishers : 山口大学医学会
山口医学 Volume 65 Issue 4
pp. 173 - 179
published_at 2016-11-01
Creators :
Kubo Hidefumi
Nagaoka Chisato
Kimura Yuta
Kawaoka Toru
Miyahara Makoto
Shimizu Ryouichi
Yamashita Yoshimi
Nagashima Yukiko
Yamamoto Shigeru
Nagano Hiroaki
Publishers : 山口大学医学会
山口医学 Volume 65 Issue 2
pp. 101 - 107
published_at 2016-05-01
Creators :
Yamaoka Yuko
Mori Kenji
Noguchi Norio
Matsunaga Takaharu
Sakaida Isao
Publishers : 山口大学医学会
山口医学 Volume 65 Issue 2
pp. 81 - 85
published_at 2016-05-01
Creators :
Matsui Tomohiro
Publishers : 山口大学医学会
山口医学 Volume 65 Issue 2
pp. 95 - 100
published_at 2016-05-01
Creators :
Mizoguchi Takahiro
Murakami Junichi
Sano Fumiho
Hayashi Masataro
Ueda Kazuhiro
Hamano Kimikazu
Publishers : 山口大学医学会
山口医学 Volume 65 Issue 1
pp. 39 - 50
published_at 2016-02-01
Creators :
Masamura Keiko
Publishers : 山口大学医学会
山口医学 Volume 65 Issue 1
pp. 23 - 29
published_at 2016-02-01
山口医学 Volume 65 Issue 1
pp. 15 - 22
published_at 2016-02-01
山口医学 Volume 65 Issue 1
pp. 51 - 63
published_at 2016-02-01
山口医学 Volume 65 Issue 1
pp. 65 - 69
published_at 2016-02-01
Creators :
Yoshimine Sota
Murakami Junichi
Sano Fumiho
Hayashi Masataro
Ueda Kazuhiro
Hamano Kimikazu
Publishers : 山口大学医学会
山口医学 Volume 65 Issue 1
pp. 5 - 13
published_at 2016-02-01
Creators :
Murakami Kyoko
Publishers : 山口大学医学会
The bulletin of the Yamaguchi Medical School Volume 63 Issue 1-2
pp. 17 - 24
published_at 2016
Aims: To clarify the usefulness of preservative-free ophthalmic preparations equipped with a filter. Methods: A total of 1,615 samples of in-use ophthalmic preparations were examined for microbial contamination. Results: Of 1,094 samples of preservative-containing ophthalmic preparations, 31 (2.8%) showed microbial contamination. Of 289 samples of preservative-free ophthalmic preparations without a filter, 6 (2.1%) were contaminated, consisting of 4 (13.8%) of 29 samples of hospital preparations and 2 (0.8%) of 260 samples of commercially available new quinolone antimicrobial agents. On the other hand, the microbial contamination rate in preservative-free ophthalmic preparations equipped with a filter was 0% (0 of 232 samples).The major contaminants detected in these preservative-containing ophthalmic preparations and preservative-free ophthalmic preparations without a filter were glucose-nonfermentative Gram-negative bacilli such as Pseudomonas fluorescens, Acinetobacter spp., and P. aeruginosa, coagulase (-) staphylococci, and Candida spp. The contaminant level was 10-99 colony forming units (CFU)/mL in 37.8% (14 of 37 samples), and 10^2 - 10^6 CFU/mL in 62.2% (23 of 37 samples). Conclusions: Preservative-free ophthalmic preparations equipped with a filter not only have zero risk of the oculotoxic effects of preservatives, but are also safe in terms of their lack of microbial contamination.
Creators :
Saisyo Atsuyuki
Oie Shigeharu
Kimura Kazuhiro
Sonoda Koh-Hei
Furukawa Hiroyuki
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 63 Issue 1-2
pp. 1 - 4
published_at 2016
Creators :
Nakamura Yoshitaka
Nakai Akira
Muto Masahiko
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 63 Issue 1-2
pp. 25 - 34
published_at 2016
Background: Angiotensin II (AngII) increases reactive oxygen species (ROS) and induces glomerular sclerosis. Toll-like receptor 4 (TLR4)-mediated inflammation enhances the renal impairment in renal inflammatory diseases. The relationship between TLR4 and AngII-induced glomerular sclerosis is unknown.Methods: Mice lacking TLR4 function (Tlr4^{lps-d}) and wild-type (WT) mice were randomized into groups treated with AngII, norepinephrine (NE) or a sub-depressor dose of the AngII receptor blocker irbesartan along with AngII for 2 weeks. We then assessed the expressions of NADPH oxidase and monocyte chemoattractant protein-1 (MCP-1) and the inflammatory cell recruitment in the glomeruli. We also evaluated the mesangial matrix proliferation and ROS.Results: AngII and NE equally increased the systolic blood pressure compared to the control mice (p<0.05). In the WT mice treated with AngII, we observed glomerular sclerosis, an increase in NADPH oxidase, MCP-1 and the infiltration of macrophages as well as ROS content in the glomeruli compared to the control mice (p<0.05), whereas the Tlr4^{lps-d} mice showed little effects of AngII on these indices. In addition, the sub-depressor-dose irbesartan treatment reversed these changes. NE had little effects on these indices. Conclusions: TLR4 plays an important role in AngII-induced oxidative stress, inflammation and glomerular sclerosis through the AT1 receptor.
Creators :
Okamoto Tadashi
Umemoto Seiji
Yoshimura Koichi
Sakumura Toshihiro
Murata Tomoaki
Fukai Tohru
Yano Masafumi
Matsuzaki Masanori
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 63 Issue 1-2
pp. 5 - 16
published_at 2016
We investigated the relationship between myocardial oxidative stress and cardiac sympathetic hyperactivity in patients with takotsubo cardiomyopathy (TC) compared with acute anteroseptal myocardial infarction (AMI). Methods: In 10 TC patients and 10 AMI patients, electrocardiogram, echocardiography, cardiac catheterization were conducted, and plasma catecholamines and urinary (U) 8-hydroxy-2’-deoxyguanosine (8-OHdG) as a marker of oxidative DNA damage were taken for one week from onset. Results: On admission, the coronary sinus (CS) had significantly higher norepinephrine (NE) and 8-OHdG levels than the aortic root (Ao) and peripheral blood vessels. Circulating catecholamines in TC patients tended to be higher than those in AMI patients
Creators :
Oda Seiko
Kobayashi Shigeki
Nanno Takuma
Ishiguchi Hironori
Myoren Takeki
Murakami Wakako
Mochizuki Mamoru
Oda Tetsuro
Okuda Shinichi
Yamada Jutaro
Okamura Takayuki
Matsuzaki Masanori
Yano Masafumi
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 63 Issue 3-4
pp. 41 - 48
published_at 2016
Creators :
Nakamura Akiko
Nakamura Yoshitaka
Tanaka Akemi
Muto Masahiko
Publishers : Yamaguchi University School of Medicine
耳鼻咽喉科免疫アレルギー Volume 33 Issue 3
pp. 201 - 204
published_at 2015-09
山口医学 Volume 64 Issue 3
pp. 177 - 181
published_at 2015-08-01
山口医学 Volume 64 Issue 3
pp. 199 - 203
published_at 2015-08-01
Creators :
Kurata Yusuke
Wakamatu Kenya
Nakamura Yoshitaka
Ichimiya Makoto
Muto Masahiko
Publishers : 山口大学医学会
山口医学 Volume 64 Issue 3
pp. 213 - 216
published_at 2015-08-01
Creators :
Okinaka Yosuke
Sugahara Kazuma
Tsuda Junko
Tanaka Kuniyoshi
Kanagawa Eiju
Shimogori Hiroaki
Yamashita Hiroshi
Publishers : 山口大学医学会
山口医学 Volume 64 Issue 3
pp. 183 - 189
published_at 2015-08-01
山口医学 Volume 64 Issue 2
pp. 87 - 99
published_at 2015-05-01
山口医学 Volume 64 Issue 2
pp. 69 - 78
published_at 2015-05-01
山口医学 Volume 64 Issue 2
pp. 139 - 143
published_at 2015-05-01
山口医学 Volume 64 Issue 2
pp. 79 - 85
published_at 2015-05-01
山口医学 Volume 64 Issue 2
pp. 145 - 152
published_at 2015-05-01
Duodenal varices(DV)are the most common of ectopic varices. Although bleeding from DV is rare, it is difficult to control bleeding and sometimes fatal. We have encountered four clinical cases of ruptured DV. Case 1:A man in his 80s presented with a history of partial gastrectomy with Billroth-II reconstruction and LC due to chronic hepatitis C. We performed single-balloon endoscopy and injected 67% N-butyl-2-cyanoacrylate(NBCA)for DV on the afferent loop with red plug. Case 2:A woman in her 40s with primary biliary cirrhosis complained of tarry stool and anemia. We performed endoscopic injection sclerotherapy(EIS)with 67% NBCA for spurting bleeding point in duodenum. Case 3:A man in his 50s with liver cirrhosis(LC)due to chronic hepatitis B complained of tarry stool. We performed endoscopic variceal ligation(EVL)and balloon-occluded retrograde transvenous obliteration(B-RTO)for DV. Case 4:A man in his 60s with alcoholic LC complained of tarry stool. We performed EIS with 67% NBCA for DV. We added EVL, clipping, and argon plasma coagulation after EIS to control bleeding. To control bleeding was achieved in all cases.
Creators :
Sasaki Ryo
Matsuda Takashi
Aibe Yuki
Nakashima Takao
Shiratsuki Shogo
Iwamoto Takuya
Ishikawa Tsuyoshi
Terai Shuji
Sakaida Isao
Publishers : 山口大学医学会
Nature communications Volume 6
pp. 6580 -
published_at 2015-03-12
Creators :
Tan Ke
Fujimoto Mitsuaki
Takii Ryosuke
Takaki Eiichi
Hayashida Naoki
Nakai Akira
Publishers : Nature Publishing Group
山口医学 Volume 64 Issue 1
pp. 11 - 16
published_at 2015-02-01
山口医学 Volume 64 Issue 1
pp. 47 - 51
published_at 2015-02-01
Creators :
Yasuda Mayumi
Okamoto Takeshi
Nagao Misato
Sasaki Sho
Nishimura Junichi
Nakamura Munetaka
Nishikawa Jun
Iida Michihisa
Yoshino Shigefumi
Sakaida Isao
Publishers : 山口大学医学会
山口医学 Volume 64 Issue 1
pp. 53 - 57
published_at 2015-02-01
A 36-year-old man diagnosed with Charcot-Marie-Tooth(CMT)disease type 1B was treated in the department of neurology of this hospital. Due to a liver injury(AST 237IU/ml, ALT 496IU/ml)which occurred in November 200X, he was introduced to our department. As a result of scrutiny, we diagnosed him with chronic hepatitis C genotype 2a(HCV-RNA 5.4logIU/ml).He will be admitted to the hospital for Interferon(IFN)therapy in April next year. Although the mechanism is unknown, there are a few reports of peripheral neuropathy related to IFN therapy. Therefore, IFN therapy is generally unfavorable for patients with neuromuscular disorders. There are several IFN preparations used in Japan. IFN alfa associated peripheral neuropathy appears less than 0.1-5% of the time. While there is no report of peripheral neuropathy related to IFN beta, we performed IFN beta and Rivabirin combination therapy with a severe follow-up by a neurologist. We could accomplish treatment without the exacerbation of neurologic symptoms. He achieved sustained viral response. When IFN therapy was provided for chronic hepatitis B or chronic hepatitis C complicated with peripheral neuropathy, there is some possibility of performing antiviral therapy safely by using IFN beta.
Creators :
Matsuura Keiji
Hidaka Isao
Hanazono Tadasuke
Saeki Issei
Iwamoto Takuya
Ishikawa Tsuyoshi
Takami Taro
Kawai Motoharu
Terai Shuji
Takashi Takashi
Sakaida Isao
Publishers : 山口大学医学会
山口医学 Volume 64 Issue 1
pp. 35 - 40
published_at 2015-02-01
Spontaneous rupture of hepatocellular carcinoma(HCC)is well known. However, rupture of HCC after transcatheter arterial chemoembolization(TACE)is a rare but potentially fatal complication. Herein, we report a case of ruptured HCC after TACE in a 73-year-old man with chronic liver injury(non-B and non-C).He had previously undergone subsegmental resection of S7 of the liver for HCC. One year later, multiple HCCs were detected. TACE was performed for the recurrent HCCs located on the surface of S2 of the liver. Epigastric pain and decreased blood pressure were observed after the treatment. Although his symptoms improved immediately, the patient gradually became anemic. Radiological examination after TACE revealed a hematoma around the S2 lesion. Therefore, we diagnosed rupture of HCC. We performed TAE and could control the bleeding. Few possible mechanisms of ruptured HCC after TACE include increasing pressure inside the tumor and capsular injury due to TACE. Consequently, HCC which has a high risk of rupture after TACE should be assured embolization and careful observation.
Creators :
Fukui Yumi
Saeki Issei
Hanazono Tadasuke
Tanabe Norikazu
Urata Yohei
Hidaka Isao
Terai Shuji
Sakaida Isao
Publishers : 山口大学医学会
山口医学 Volume 64 Issue 1
pp. 41 - 45
published_at 2015-02-01
Creators :
Nishimura Tatsuro
Hashimoto Shinichi
Kawasato Ryo
Shirasawa Tomohiro
Yokota Yasuyuki
Shibata Taimei
Okamoto Takeshi
Nishikawa Jun
Sakaida Isao
Publishers : 山口大学医学会
山口医学 Volume 64 Issue 1
pp. 5 - 9
published_at 2015-02-01
Creators :
Okano Kozue
Publishers : 山口大学医学会
Molecular and cellular biology Volume 35 Issue 1
pp. 11 - 25
published_at 2015-01-01
Creators :
Takii Ryosuke
Fujimoto Mitsuaki
Tan Ke
Takaki Eiichi
Hayashida Naoki
Nakato Ryuichiro
Shirahige Katsuhiko
Nakai Akira
Publishers : American Society for Microbiology
The bulletin of the Yamaguchi Medical School Volume 62 Issue 1-2
pp. 1 - 10
published_at 2015
Creators :
Ogino Yasuaki
Kaneda Kotaro
Nakahara Takashi
Todani Masaki
Miyauchi Takashi
Fujita Motoki
Kawamura Yoshikatsu
Oda Yasutaka
Tsuruta Ryosuke
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 62 Issue 3-4
pp. 59 - 65
published_at 2015
Creators :
Katsura Shunsaku
Enoki Tadahiko
Kugimiya Naruji
Takemoto Yoshihiro
Oka Kazuhito
Harada Eijiro
Hamano Kimikazu
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 62 Issue 3-4
pp. 29 - 36
published_at 2015
Creators :
Yanai Ryoji
Sonoda Koh-Hei
Connor Kip M.
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 62 Issue 1-2
pp. 11 - 19
published_at 2015
Creators :
Ueda Takaaki
Tokuda Osamu
Harada Yuko
Iida Etsushi
Shirasawa Kenzo
Yamashita Akihisa
Hosoda Bungo
Matsunaga Naofumi
Publishers : Yamaguchi University School of Medicine
The bulletin of the Yamaguchi Medical School Volume 62 Issue 3-4
pp. 37 - 45
published_at 2015
Bioscience, biotechnology, and biochemistry Volume 79 Issue 4
pp. 587 - 597
published_at 2015
Creators :
Nga Bui Thi To
Luziga Claudius
Yamamoto Misa
Kusakabe Ken Takeshi
Yamamoto Yoshimi
Publishers : 日本農芸化学会
Cancer Letters Volume 354 Issue 2
pp. 329 - 335
published_at 2014-11-28
Creators :
Nakamura Yoshitaka
Fujimoto Mitsuaki
Fukushima Sonoko
Nakamura Akiko
Hayashida Naoki
Takii Ryosuke
Takaki Eiichi
Nakai Akira
Muto Masahiko
Publishers : Elsevier